School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, China.
Guangzhou Customs Technology Centre, Guangzhou, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2022 Nov;39(11):1773-1784. doi: 10.1080/19440049.2022.2118864. Epub 2022 Sep 7.
, as an expensive traditional Chinese medicine and edible fungus mycelium, lacks an effective quality evaluation method, especially and cultivated . In this study, a feasible workflow method was developed for traceability evaluation of wild and cultivated based on mass spectrometry-based metabolomics. Mass spectrometry data were firstly acquired from samples by liquid chromatography-quadrupole and time of flight mass spectrometry. Characteristic mass spectrometry peaks were extracted by applying the MZmine. Then significant markers were obtained from samples by orthogonal partial least square discriminant analysis. Then, identification of significant markers were identified by MS-FINDER data analytics. The results showed that Changdu, the other four wild origins (Naqu, Xinghai, Yushu and Guoluo) and cultivated samples could be significantly distinguished. This identified significant markers of , including 174 special significant markers for the wild samples, 204 special significant markers for the cultivated samples and 87 share significant markers. Number of 87 shared significant markers were identified in the wild and cultivated , especially 28 confident significant compounds, such as adenosine, riboflavin, tyrosine, arginine and glutamine. These shared significant markers might support the quality control of multi-targets of , compared with a single target in the Chinese Pharmacopoeia. The special significant markers indicated that cultivated was different from the wild based on mass spectrometry-based metabolomics. In the comparison of chromatographic fingerprint technology, it was found that the established feasible workflow method was easy to acquire significant markers and traceability of . This feasible workflow method has great potential to be successful for comprehensive and traceability evaluation of the wild and cultivated
作为一种昂贵的中药和食用真菌菌丝体，缺乏有效的质量评价方法，特别是野生和栽培的。本研究基于基于质谱的代谢组学，为野生和栽培建立了一种可行的可追溯性评估方法。通过液相色谱-四极杆和飞行时间质谱对样品进行质谱数据采集。通过 MZmine 提取特征质谱峰。然后通过正交偏最小二乘判别分析从样品中获得显著标记物。然后，通过 MS-FINDER 数据分析鉴定显著标记物。结果表明，昌都、其他四个野生起源地（那曲、星海、玉树和果洛）和栽培样本可以明显区分。这鉴定了的显著标记物，包括野生样本的 174 个特殊显著标记物、栽培样本的 204 个特殊显著标记物和 87 个共享显著标记物。在野生和栽培中鉴定了 87 个共享显著标记物，特别是 28 种有信心的显著化合物，如腺苷、核黄素、酪氨酸、精氨酸和谷氨酰胺。与《中国药典》中单一目标相比，这些共享的显著标记物可能支持的多目标质量控制。特殊的显著标记物表明，栽培的与野生的基于基于质谱的代谢组学不同。在色谱指纹图谱技术的比较中，发现建立的可行工作流程方法易于获取显著标记物和野生和栽培的可追溯性。该可行的工作流程方法具有很大的潜力，可成功用于综合和可追溯性评估野生和栽培的。
