热失活和大规模裂解对实时逆转录聚合酶链反应检测 SARS-CoV-2 的影响：一项实验研究。
Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study.
机构信息
HIV/AIDS Disease Research Team, TB and HIV/AIDS Disease Research Directorate, Ethiopian PublicHealth Institute, Addis Ababa, Ethiopia.
Department of Medical Laboratory Sciences, College of Health Sciences, AddisAbaba University, Addis Ababa, Ethiopia.
出版信息
BMC Res Notes. 2022 Sep 7;15(1):295. doi: 10.1186/s13104-022-06184-z.
OBJECTIVE
This study aimed to investigate the effect of heat inactivation and chemical bulklysis on SARS-CoV-2 detection.
RESULTS
About 6.2% (5/80) of samples were changed to negative results in heat inactivation at 60 °C and about 8.7% (7/80) of samples were changed to negative in heat inactivation at 100 °C. The Ct values of heat-inactivated samples (at 60 °C, at 100 °C, and bulk lysis) were significantly different from the temperature at 56 °C. The effect of heat on Ct value should be considered when interpreting diagnostic PCR results from clinical samples which could have an initial low virus concentration. The efficacy of heat-inactivation varies greatly depending on temperature and duration. Local validation of heat-inactivation and its effects is therefore essential for molecular testing.
目的
本研究旨在探讨热失活和化学裂解对 SARS-CoV-2 检测的影响。
结果
约 6.2%（5/80）的样本在 60°C 热失活时变为阴性结果，约 8.7%（7/80）的样本在 100°C 热失活时变为阴性结果。热失活样本（60°C、100°C 和裂解）的 Ct 值与 56°C 时的温度显著不同。当解释临床样本的诊断性 PCR 结果时，应考虑热对 Ct 值的影响，这些样本可能初始病毒浓度较低。热失活的效果因温度和持续时间而异，因此对热失活及其影响进行本地验证对于分子检测至关重要。
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