Department of Intensive Care Units, Affiliated Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian, China.
Department of Internal Medicine, The Affiliated Zhong Shan Hospital of Dalian University, No. 6 Jiefang Street, Dalian, China.
J Int Med Res. 2022 Sep;50(9):3000605221118680. doi: 10.1177/03000605221118680.
Sepsis is a systemic and deleterious host reaction to severe infection. Cardiac dysfunction is an established serious outcome of multiorgan failure associated with this condition. Therefore, it is important to develop drugs targeting sepsis-induced cardiac damage and inflammation. Thymoquinone (TQ) has anti-inflammatory, anti-oxidant, anti-fibrotic, anti-tumor, and anti-apoptotic effects. This study examined the effects of thymoquinone on sepsis-induced cardiac damage.
Male BALB/c mice were randomly segregated into four groups: control, TQ, cecal ligation and puncture (CLP), and CLP + TQ groups. CLP was performed after gavaging the mice with TQ for 2 weeks. After 48 hours, we estimated the histopathological changes in the cardiac tissue and the serum levels of cardiac troponin-T. We evaluated the expression of factors associated with inflammation, apoptosis, oxidative stress, and the PI3K/AKT pathway.
TQ significantly reduced intestinal histological alterations and inhibited the upregulation of interleukin-6, tumor necrosis factor-α, Bax, NOX4, p-PI3K, and p-AKT. TQ also increased Bcl-2, HO-1, and NRF2 expression.
These results suggest that TQ effectively modulates pro-inflammatory, apoptotic, oxidative stress, and PI3K/AKT pathways, making it indispensable in the treatment of sepsis-induced cardiac damage.
败血症是一种严重感染导致的全身有害宿主反应。心功能障碍是与这种情况相关的多器官衰竭的严重后果之一。因此，开发针对败血症引起的心脏损伤和炎症的药物非常重要。百里醌（TQ）具有抗炎、抗氧化、抗纤维化、抗肿瘤和抗凋亡作用。本研究探讨了百里醌对败血症引起的心脏损伤的影响。
雄性 BALB/c 小鼠随机分为四组：对照组、TQ 组、盲肠结扎穿孔（CLP）组和 CLP+TQ 组。CLP 前两周对小鼠进行 TQ 灌胃。48 小时后，我们评估了心脏组织的组织病理学变化和血清中心肌肌钙蛋白-T 水平。我们评估了与炎症、细胞凋亡、氧化应激和 PI3K/AKT 通路相关的因子的表达。
TQ 显著减轻了肠道组织学改变，并抑制了白细胞介素-6、肿瘤坏死因子-α、Bax、NOX4、p-PI3K 和 p-AKT 的上调。TQ 还增加了 Bcl-2、HO-1 和 NRF2 的表达。
这些结果表明 TQ 可有效调节促炎、凋亡、氧化应激和 PI3K/AKT 通路，使其成为治疗败血症引起的心脏损伤不可或缺的药物。
