Long Dingde, Chen Yayu, Qu Liangchao, Dong Yang
Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China.
Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China.
Oncol Lett. 2022 Aug 17;24(4):347. doi: 10.3892/ol.2022.13467. eCollection 2022 Oct.
As a gynecological malignancy, endometrial cancer (EC) has a high incidence and mortality rate in women. The aim of the present study was to investigate the mechanism of EC and to identify novel effective treatment methods for this disease. The viability and proliferation of the RL95-2 human endometrial cancer cell line were assessed using Cell Counting Kit-8 assays. Colony formation, wound healing, Transwell, TUNEL and immunofluorescence assays were used to assess the effects of 5, 10 and 15 mM lidocaine on the colony formation, migration, invasiveness, apoptosis and Beclin 1 protein expression of RL95-2 cells, respectively. Furthermore, western blotting was used to analyze the protein expression levels of apoptosis- and autophagy-related proteins. The results demonstrated that lidocaine inhibited the viability, proliferation and migration of EC cells, and promoted apoptosis. Furthermore, lidocaine was demonstrated to induce autophagy and Beclin 1 protein expression in EC cells. In conclusion, lidocaine inhibited the proliferation and migration of EC cells, and promoted apoptosis via autophagy induction, which indicated that lidocaine may be a potential therapeutic drug for the treatment of EC.
作为一种妇科恶性肿瘤，子宫内膜癌（EC）在女性中具有较高的发病率和死亡率。本研究的目的是探讨子宫内膜癌的发病机制，并确定针对该疾病的新型有效治疗方法。使用细胞计数试剂盒-8（Cell Counting Kit-8）检测法评估RL95-2人子宫内膜癌细胞系的活力和增殖情况。分别采用集落形成、伤口愈合、Transwell、TUNEL和免疫荧光检测法评估5 mM、10 mM和15 mM利多卡因对RL95-2细胞集落形成、迁移、侵袭、凋亡及Beclin 1蛋白表达的影响。此外，采用蛋白质印迹法分析凋亡和自噬相关蛋白的表达水平。结果表明，利多卡因抑制子宫内膜癌细胞的活力、增殖和迁移，并促进其凋亡。此外，利多卡因可诱导子宫内膜癌细胞发生自噬并使Beclin 1蛋白表达增加。总之，利多卡因通过诱导自噬抑制子宫内膜癌细胞的增殖和迁移，并促进其凋亡，这表明利多卡因可能是一种治疗子宫内膜癌的潜在治疗药物。
