Li Tao, Deng Yunshuang, Huang Jiaquan, Liang Jiayin, Zheng Yongqin, Xu Qian, Fan Shuting, Li Wenting, Deng Xiaoling, Zheng Zheng
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China.
Front Plant Sci. 2022 Aug 22;13:980033. doi: 10.3389/fpls.2022.980033. eCollection 2022.
The holoparasitic dodder ( spp.) is able to transfer mRNA and certain plant pathogens (e.g., viruses and bacteria) from the host plant. " Liberibacter asiaticus," the phloem-limited causative agent of citrus Huanglongbing, can be transferred from citrus to periwinkle () mediated by dodder. However, characterization of mRNA transport between dodder and citrus/periwinkle remains unclear. In this study, we sequenced transcriptomes of dodder and its parasitizing host, sweet orange ( "Newhall") and periwinkle (), to identify and characterize mRNA transfer between dodder and the host plant during parasitism. The mRNA transfer between dodder and citrus/periwinkle was bidirectional and most of the transfer events occurred in the interface tissue. Compared with the citrus-dodder system, mRNA transfer in the periwinkle-dodder system was more frequent. Function classification revealed that a large number of mRNAs transferred between dodder and citrus/periwinkle were involved in secondary metabolism and stress response. Dodder transcripts encoding proteins associated with microtubule-based processes and cell wall biogenesis were transferred to host tissues. In addition, transcripts involved in translational elongation, plasmodesmata, and the auxin-activated signaling pathway were transmitted between dodder and citrus/periwinkle. In particular, transcripts involved in shoot system development and flower development were transferred between the host and dodder in both directions. The high abundance of dodder-origin transcripts, encoding MIP aquaporin protein, and -adenosylmethionine synthetase 1 protein, in citrus and periwinkle tissues indicated they could play an important biological role in dodder-host interaction. In addition, the uptake of host mRNAs by dodder, especially those involved in seed germination and flower development, could be beneficial for the reproduction of dodder. The results of this study provide new insights into the RNA-based interaction between dodder and host plants.
全寄生菟丝子（菟丝子属）能够从寄主植物中转移信使核糖核酸（mRNA）和某些植物病原体（如病毒和细菌）。“亚洲韧皮杆菌”是柑橘黄龙病的韧皮部限制病原体，可由菟丝子介导从柑橘转移到长春花。然而，菟丝子与柑橘/长春花之间mRNA转运的特征仍不清楚。在本研究中，我们对菟丝子及其寄生寄主纽荷尔脐橙和长春花的转录组进行了测序，以鉴定和表征寄生过程中菟丝子与寄主植物之间的mRNA转移。菟丝子与柑橘/长春花之间的mRNA转移是双向的，且大多数转移事件发生在界面组织中。与柑橘 - 菟丝子系统相比，长春花 - 菟丝子系统中的mRNA转移更为频繁。功能分类显示，菟丝子与柑橘/长春花之间转移的大量mRNA参与次生代谢和应激反应。编码与基于微管的过程和细胞壁生物合成相关蛋白质的菟丝子转录本被转移到寄主组织中。此外，参与翻译延伸、胞间连丝和生长素激活信号通路相关的转录本在菟丝子与柑橘/长春花之间进行了传递。特别是，参与茎系统发育和花发育的转录本在寄主和菟丝子之间双向转移。柑橘和长春花组织中高丰度的菟丝子来源转录本，编码水通道蛋白MIP和S - 腺苷甲硫氨酸合成酶1蛋白，表明它们可能在菟丝子 - 寄主相互作用中发挥重要生物学作用。此外，菟丝子摄取寄主mRNA，尤其是那些参与种子萌发和花发育的mRNA，可能有利于菟丝子的繁殖。本研究结果为菟丝子与寄主植物之间基于RNA的相互作用提供了新的见解。
