Song Juan, Bian Jinge, Xue Na, Xu Yuxing, Wu Jianqiang
Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China.
Plant Divers. 2021 Apr 20;44(1):1-10. doi: 10.1016/j.pld.2021.03.004. eCollection 2022 Jan.
mRNAs are transported within a plant through phloem. Aphids are phloem feeders and dodders ( spp.) are parasites which establish phloem connections with host plants. When aphids feed on dodders, whether there is trafficking of mRNAs among aphids, dodders, and host plants and if aphid feeding affects the mRNA transfer between dodders and hosts are unclear. We constructed a green peach aphid (GPA, )-dodder ()-cucumber () tritrophic system by infesting GPAs on , which parasitized cucumber hosts. We found that GPA feeding activated defense-related phytohormonal and transcriptomic responses in both and cucumbers and large numbers of mRNAs were found to be transferred between and cucumbers and between and GPAs; importantly, GPA feeding on greatly altered inter-species mobile mRNA profiles. Furthermore, three cucumber mRNAs and three GPA mRNAs could be respectively detected in GPAs and cucumbers. Moreover, our statistical analysis indicated that mRNAs with high abundances and long transcript lengths are likely to be mobile. This study reveals the existence of inter-species and even inter-kingdom mRNA movement among insects, parasitic plants, and parasite hosts, and suggests complex regulation of mRNA trafficking.
信使核糖核酸（mRNAs）在植物体内通过韧皮部运输。蚜虫以韧皮部为食，菟丝子（菟丝子属）是与寄主植物建立韧皮部连接的寄生虫。当蚜虫以菟丝子为食时，蚜虫、菟丝子和寄主植物之间是否存在信使核糖核酸的运输，以及蚜虫取食是否会影响菟丝子和寄主之间的信使核糖核酸转移尚不清楚。我们通过在寄生黄瓜寄主的菟丝子上接种绿色桃蚜（GPA）构建了一个绿色桃蚜-GPA-菟丝子-黄瓜的三营养体系。我们发现，GPA取食激活了菟丝子和黄瓜中与防御相关的植物激素和转录组反应，并且发现大量信使核糖核酸在菟丝子和黄瓜之间以及菟丝子和GPA之间转移；重要的是，GPA取食菟丝子极大地改变了种间移动信使核糖核酸的图谱。此外，在GPA和黄瓜中分别检测到三种黄瓜信使核糖核酸和三种GPA信使核糖核酸。此外，我们的统计分析表明，高丰度和长转录本长度的信使核糖核酸可能具有移动性。这项研究揭示了昆虫、寄生植物和寄生虫寄主之间存在种间甚至跨王国的信使核糖核酸移动，并表明信使核糖核酸运输存在复杂的调控。
