Nagy Lilla, Rauch Boglárka, Szerafin Tamás, Uray Karen, Tóth Attila, Bai Péter
Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Department of Cardiology and Heart Surgery, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Front Cell Dev Biol. 2022 Aug 22;10:979330. doi: 10.3389/fcell.2022.979330. eCollection 2022.
Beige adipocytes play key roles in organismal energy and metabolic balance. In this study, we assessed whether the supplementation of human white adipocytes, differentiated from human adipose tissue-derived stem cells, with nicotinamide riboside (NR), a potent NAD + precursor, can shift differentiation to beige adipocytes (beiging). NR induced mitochondrial biogenesis and the expression of beige markers (TBX1 and UCP1) in white adipocytes demonstrating that NR can declutch beiging. NR did not induce PARP activity but supported SIRT1 induction, which plays a key role in beiging. NR induced etomoxir-resistant respiration, suggesting increases in the oxidation of carbohydrates, carbohydrate breakdown products, or amino acids. Furthermore, NR boosted oligomycin-resistant respiration corresponding to uncoupled respiration. Enhanced etomoxir and oligomycin-resistant respiration were dependent on mitochondrial reactive-species production. Taken together, NR supplementation can induce beiging and uncoupled respiration, which are beneficial for combatting metabolic diseases.
米色脂肪细胞在机体能量和代谢平衡中发挥着关键作用。在本研究中，我们评估了用烟酰胺核糖（NR）（一种有效的NAD⁺前体）补充从人脂肪组织衍生干细胞分化而来的人白色脂肪细胞，是否能使分化转变为米色脂肪细胞（米色化）。NR诱导白色脂肪细胞中线粒体生物发生和米色标志物（TBX1和UCP1）的表达，表明NR可引发米色化。NR未诱导PARP活性，但支持SIRT1诱导，而SIRT1在米色化中起关键作用。NR诱导依托莫昔芬抗性呼吸，提示碳水化合物、碳水化合物分解产物或氨基酸的氧化增加。此外，NR增强了与解偶联呼吸相对应的寡霉素抗性呼吸。增强的依托莫昔芬和寡霉素抗性呼吸依赖于线粒体活性氧的产生。综上所述，补充NR可诱导米色化和解偶联呼吸，这对对抗代谢疾病有益。
