Tools for Bio-Imaging, Max-Planck-Institute for Biological Intelligence (i.F.), Martinsried, Germany.
J Physiol. 2023 Oct;601(19):4217-4226. doi: 10.1113/JP279510. Epub 2022 Sep 30.
Calcium in interstitial fluids is a crucial ion pool for entry into cells through a plethora of calcium-permeable channels. It is also sensed actively by dedicated receptors. While the mechanisms of global calcium homeostasis and regulation in body fluids appear well understood, more efforts and new technology are needed to elucidate local calcium handling in the small and relatively isolated interstitial spaces between cells. Here we review current methodology for monitoring interstitial calcium and highlight the potential of new approaches for its study. In particular, new generations of high-performance low-affinity genetically encoded calcium indicators could allow imaging of calcium in relatively inaccessible intercellular structures in live tissues and organisms.
细胞间隙液中的钙是细胞内钙离子进入细胞的关键离子池，通过多种钙离子通透性通道进入细胞。钙离子也可以被专门的受体主动感知。虽然体液中钙的整体稳态和调节机制似乎已经得到很好的理解，但仍需要更多的努力和新技术来阐明细胞间相对较小且相对隔离的细胞间隙中局部钙处理。本文综述了监测细胞间钙的现有方法，并强调了新方法研究钙的潜力。特别是新一代高性能、低亲和力的基因编码钙指示剂可以实现活体组织和生物体内相对难以进入的细胞间结构中钙的成像。
