光遗传学控制电压门控钙通道。
Optogenetic Control of Voltage-Gated Calcium Channels.
机构信息
Institute of Biosciences and Technology, College of Medicine, Texas A&M University, 2121 W Holcombe Blvd, Houston, TX, 77030, USA.
Beijing Key Laboratory of Gene Resource and Molecular, Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China.
出版信息
Angew Chem Int Ed Engl. 2018 Jun 11;57(24):7019-7022. doi: 10.1002/anie.201713080. Epub 2018 Apr 16.
Voltage-gated Ca (Ca ) channels mediate Ca entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded Ca channel modulators, to enable photo-tunable modulation of Ca channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for Ca channels could find broad applications in interrogating a wide range of Ca -mediated physiological processes.
电压门控钙 (Ca ) 通道介导 Ca 内流进入可兴奋细胞，以调节膜去极化后无数的细胞事件。我们报告了 RGK GTPases 的工程改造，这是一类遗传编码的 Ca 通道调节剂，可实现可兴奋哺乳动物细胞中 Ca 通道活性的光调控。这种专门为 Ca 通道设计的光遗传学工具（命名为 optoRGK）可以广泛应用于研究广泛的 Ca 介导的生理过程。
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