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光遗传学控制电压门控钙通道。

Optogenetic Control of Voltage-Gated Calcium Channels.

机构信息

Institute of Biosciences and Technology, College of Medicine, Texas A&M University, 2121 W Holcombe Blvd, Houston, TX, 77030, USA.

Beijing Key Laboratory of Gene Resource and Molecular, Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China.

出版信息

Angew Chem Int Ed Engl. 2018 Jun 11;57(24):7019-7022. doi: 10.1002/anie.201713080. Epub 2018 Apr 16.

DOI:10.1002/anie.201713080
PMID:29569306
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6032918/
Abstract

Voltage-gated Ca (Ca ) channels mediate Ca entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded Ca channel modulators, to enable photo-tunable modulation of Ca channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for Ca channels could find broad applications in interrogating a wide range of Ca -mediated physiological processes.

摘要

电压门控钙 (Ca ) 通道介导 Ca 内流进入可兴奋细胞，以调节膜去极化后无数的细胞事件。我们报告了 RGK GTPases 的工程改造，这是一类遗传编码的 Ca 通道调节剂，可实现可兴奋哺乳动物细胞中 Ca 通道活性的光调控。这种专门为 Ca 通道设计的光遗传学工具（命名为 optoRGK）可以广泛应用于研究广泛的 Ca 介导的生理过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5c1/6032918/c2ab4758f3e6/ANIE-57-7019-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5c1/6032918/68f8a674424f/ANIE-57-7019-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5c1/6032918/2470100e6328/ANIE-57-7019-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5c1/6032918/98f3149ef52e/ANIE-57-7019-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5c1/6032918/c2ab4758f3e6/ANIE-57-7019-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5c1/6032918/68f8a674424f/ANIE-57-7019-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5c1/6032918/2470100e6328/ANIE-57-7019-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5c1/6032918/98f3149ef52e/ANIE-57-7019-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5c1/6032918/c2ab4758f3e6/ANIE-57-7019-g003.jpg

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1
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Cell Calcium. 2017 Jun;64:36-46. doi: 10.1016/j.ceca.2017.01.004. Epub 2017 Jan 16.
2
Sensitive red protein calcium indicators for imaging neural activity.用于神经活动成像的灵敏红色蛋白质钙指示剂。
Elife. 2016 Mar 24;5:e12727. doi: 10.7554/eLife.12727.
3
Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation.Ca(2+)信号的近红外光可激活控制与光遗传学免疫调节
可编程合成受体：下一代细胞和基因治疗。
Signal Transduct Target Ther. 2024 Jan 3;9(1):7. doi: 10.1038/s41392-023-01680-5.
4
Cardiac optogenetics: shining light on signaling pathways.心脏光遗传学：照亮信号通路。
Pflugers Arch. 2023 Dec;475(12):1421-1437. doi: 10.1007/s00424-023-02892-y. Epub 2023 Dec 14.
5
Design and Applications of Genetically-Encoded Voltage-Dependent Calcium Channel Inhibitors.基因编码电压门控钙通道抑制剂的设计与应用。
Handb Exp Pharmacol. 2023;279:139-155. doi: 10.1007/164_2023_656.
6
Optophysiology: Illuminating cell physiology with optogenetics.光物理学生理学：用光遗传学照亮细胞生理学。
Physiol Rev. 2022 Jul 1;102(3):1263-1325. doi: 10.1152/physrev.00021.2021. Epub 2022 Jan 24.
7
Deciphering Molecular Mechanisms and Intervening in Physiological and Pathophysiological Processes of Ca Signaling Mechanisms Using Optogenetic Tools.利用光遗传学工具解析钙信号转导机制的分子机制并干预其生理和病理生理过程。
Cells. 2021 Nov 28;10(12):3340. doi: 10.3390/cells10123340.
8
Engineering Supramolecular Organizing Centers for Optogenetic Control of Innate Immune Responses.工程化超分子组织中心用于光遗传学控制先天免疫反应。
Adv Biol (Weinh). 2021 May;5(5):e2000147. doi: 10.1002/adbi.202000147. Epub 2020 Dec 30.
9
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Annu Rev Biochem. 2021 Jun 20;90:475-501. doi: 10.1146/annurev-biochem-072420-112431. Epub 2021 Mar 29.
10
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Adv Exp Med Biol. 2021;1293:73-88. doi: 10.1007/978-981-15-8763-4_5.
Elife. 2015 Dec 8;4:e10024. doi: 10.7554/eLife.10024.
4
Targeting voltage-gated calcium channels in neurological and psychiatric diseases.靶向治疗神经和精神疾病中的电压门控钙通道。
Nat Rev Drug Discov. 2016 Jan;15(1):19-34. doi: 10.1038/nrd.2015.5. Epub 2015 Nov 6.
5
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6
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7
The Physiology, Pathology, and Pharmacology of Voltage-Gated Calcium Channels and Their Future Therapeutic Potential.电压门控钙通道的生理学、病理学和药理学及其未来的治疗潜力。
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8
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Neuron. 2014 Apr 2;82(1):24-45. doi: 10.1016/j.neuron.2014.03.016.
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The voltage-gated calcium channel functions as the molecular switch of synaptic transmission.电压门控钙通道作为突触传递的分子开关起作用。
Annu Rev Biochem. 2013;82:607-35. doi: 10.1146/annurev-biochem-080411-121438. Epub 2013 Jan 17.