Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.
Department of Developmental Biology, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.
J Am Chem Soc. 2022 Sep 21;144(37):16819-16826. doi: 10.1021/jacs.2c04479. Epub 2022 Sep 8.
MicroRNAs play crucial and dynamic roles in vertebrate development and diseases. Some, like miR-430, are highly expressed during early embryo development and regulate hundreds of transcripts, which can make it difficult to study their role in the timing and location of specific developmental processes using conventional morpholino oligonucleotide (MO) knockdown or genetic deletion approaches. We demonstrate that light-activated circular morpholino oligonucleotides (cMOs) can be applied to the conditional control of microRNA function. We targeted miR-430 in zebrafish embryos to study its role in the development of the embryo body and the heart. Using 405 nm irradiation, precise spatial and temporal control over miR-430 function was demonstrated, offering insight into the cell populations and developmental timepoints involved in each process.
MicroRNAs 在脊椎动物的发育和疾病中发挥着至关重要和动态的作用。有些，如 miR-430，在早期胚胎发育过程中高度表达，调节数百个转录本，这使得使用传统的形态发生素寡核苷酸（MO）敲低或遗传缺失方法来研究它们在特定发育过程中的时间和位置的作用变得困难。我们证明，光激活的圆形形态发生素寡核苷酸（cMOs）可用于微 RNA 功能的条件控制。我们将 miR-430 靶向斑马鱼胚胎，以研究其在胚胎体和心脏发育中的作用。通过 405nm 照射，精确地控制了 miR-430 的功能，深入了解了每个过程中涉及的细胞群体和发育时间点。
