Department of Dermatology, General Hospital of Xinjiang Military Command, Urumqi, China.
Department of Nephrology, General Hospital of Xinjiang Military Command, Urumqi, China.
Mol Pain. 2022 Apr;18:17448069221128423. doi: 10.1177/17448069221128423.
Psoriasis is a common chronic skin inflammatory disease. Understanding the pathogenesis of psoriasis and identifying novel therapeutic targets are under investigation.
Gene expression profiles were obtained from GSE13355, GSE30999 and GSE54456 datasets to identify differentially expressed genes (DEGs) between psoriasis and normal controls. Enrichment analysis was used to identify the biological functions and pathways of common genes from three groups of DEGs. Protein-protein interaction (PPI) network was then constructed to identify key genes according to degree of connectivity. Expression of genes was detected by the method of qRT-PCR and immunohistochemistry. The infiltration of immune cells of psoriasis were quantified and detected by flow cytometry.
A total of 146 common genes were identified between psoriasis and normal controls. They were significantly enriched in IL-17, chemokine, and NOD-like receptor (NLR) signaling pathway. Ten key genes were selected with bigger degree of connectivity through PPI network, and ARG1 and CXCL2 had better predictive ability based on ROC curves. Increased expression of ARG1 and CXCL2 in psoriasis patients were verified by qRT-PCR and immunohistochemistry method. In addition, a lot of immune cells were upregulated in psoriasis compared to healthy controls through ssGSEA and flow cytometry.
ARG1 and CXCL2 may serve as biomarkers and potential therapy for psoriasis. This may be related to the immune response and NLR pathway.
银屑病是一种常见的慢性皮肤炎症性疾病。了解银屑病的发病机制和确定新的治疗靶点正在研究中。
从 GSE13355、GSE30999 和 GSE54456 数据集获得基因表达谱，以鉴定银屑病与正常对照之间的差异表达基因（DEG）。富集分析用于鉴定三组 DEG 中常见基因的生物学功能和途径。然后根据连通度构建蛋白质-蛋白质相互作用（PPI）网络，以识别关键基因。通过 qRT-PCR 和免疫组织化学方法检测基因的表达。通过流式细胞术定量和检测银屑病免疫细胞的浸润。
在银屑病与正常对照之间共鉴定出 146 个常见基因。它们在 IL-17、趋化因子和 NOD 样受体（NLR）信号通路中显著富集。通过 PPI 网络选择了 10 个具有较大连通度的关键基因，并且基于 ROC 曲线，ARG1 和 CXCL2 具有更好的预测能力。通过 qRT-PCR 和免疫组织化学方法验证了银屑病患者中 ARG1 和 CXCL2 的表达增加。此外，通过 ssGSEA 和流式细胞术，与健康对照相比，银屑病患者中有大量免疫细胞上调。
ARG1 和 CXCL2 可能作为银屑病的生物标志物和潜在治疗靶点。这可能与免疫反应和 NLR 途径有关。
