Department of Biochemistry, Kurukshetra University Kurukshetra, Kurukshetra, Haryana, 136119, India.
Department of Biotechnology, Indira Gandhi Meerpur University, Rewari, Haryana, India.
Mol Biol Rep. 2022 Nov;49(11):10251-10257. doi: 10.1007/s11033-022-07779-2. Epub 2022 Sep 8.
Incomplete combustion of wood releases toxic chemicals. Exposure to these chemicals during charcoal production can modulate redox status of cellular system which may further lead to genomic instability and of antioxidant enzymes. Genetic polymorphism may alter the functioning properties of these enzymes and modulate the response to oxidative stress.
In this study, we analyzed the link between genetic polymorphism and enzyme activity for antioxidant enzymes: MnSOD and GPx-1 in charcoal workers and control population. This study included 77 charcoal workers and 79 demographically matched healthy control subjects. This association was studied using multiple linear regression, adjusted for confounding factors viz. age, consumption habits and exposure duration.
SOD activity was lower for TT genotype (3.47 ± 0.66; 5.92 ± 1.08) versus CC genotype (3.47 ± 0.66; 6.67 ± 1.60) in control and charcoal workers respectively. Significant lower GPx-1 activity was found in leu/leu genotype (7.25 ± 0.38; 3.59 ± 0.57) when compared to pro/pro genotype (7.78 ± 0.59; 4.28 ± 0.71) and pro/leu genotype (8.48 ± 0.34; 4.30 ± 0.76) in control population and charcoal workers respectively. A significant difference in the levels of 1-Hydroxypyrene (biomarker of exposure) and SOD and GPx-1 activity (biomarkers of oxidative stress) was evident in exposed group in comparison to the control one.
Collectively, our findings suggested that PAH influenced the mode of action of SOD and GPx-1 which were impacted by polymorphism in SOD and GPx-1 gene. Hence, polymorphism of MnSOD and GPx-1 genes were found to play a modulatory role in human susceptibility to oxidative damage induced by wood smoke in charcoal workers.
木材不完全燃烧会释放有毒化学物质。在木炭生产过程中接触这些化学物质会调节细胞系统的氧化还原状态，这可能进一步导致基因组不稳定和抗氧化酶的产生。遗传多态性可能改变这些酶的功能特性，并调节对氧化应激的反应。
在这项研究中，我们分析了抗氧化酶 MnSOD 和 GPx-1 的遗传多态性与酶活性之间的关系，研究对象为木炭工人和对照人群。这项研究包括 77 名木炭工人和 79 名年龄、性别相匹配的健康对照者。采用多元线性回归分析，调整年龄、饮食习惯和暴露时间等混杂因素，对这种关联进行研究。
与 CC 基因型（3.47±0.66；6.67±1.60）相比，TT 基因型（3.47±0.66；5.92±1.08）的 SOD 活性在对照组和木炭工人中均较低。与 pro/pro 基因型（7.78±0.59；4.28±0.71）和 pro/leu 基因型（8.48±0.34；4.30±0.76）相比，leu/leu 基因型的 GPx-1 活性明显较低（7.25±0.38；3.59±0.57），这在对照组和木炭工人中均如此。暴露组中 1-羟基芘（暴露标志物）和 SOD、GPx-1 活性（氧化应激标志物）的水平明显高于对照组。
总之，我们的研究结果表明，多环芳烃影响 SOD 和 GPx-1 的作用模式，而 SOD 和 GPx-1 基因的多态性则影响其活性。因此，MnSOD 和 GPx-1 基因的多态性被发现可调节木炭工人因木烟引起的氧化损伤的人类易感性。
