Miglani Kanika, Kumar Sunil, Yadav Anita, Aggarwal Neeraj, Ahmad Imteyaz, Gupta Ranjan
1 Department of Biochemistry, Kurukshetra University Kurukshetra, Haryana, India.
2 Deprtment of Biotechnology, Kurukshetra University Kurukshetra, Haryana, India.
Toxicol Ind Health. 2019 Jul;35(7):486-496. doi: 10.1177/0748233719862728.
We conducted a cohort study of tandoor workers to evaluate the relationship between the biomarkers of oxidative and genotoxic damage and exposure to polyaromatic hydrocarbons. A series of oxidative and genotoxic damage biomarkers, including urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG), malondialdehyde (MDA) content, and tail moment (TM) by comet assay, was studied. A total of 76 tandoor workers and 79 demographically matched healthy individuals as controls were included. Our results showed that the tandoor workers had significantly higher urinary levels of 1-hydroxypyrene, urinary 8-OHdG, MDA content, and TM compared with the control population. The concentration of all these biomarkers increased with age in the control population as well as tandoor workers. In tandoor workers, significant variation in MDA, 8-oxodG (8-oxo-2'-deoxyguanosine) and TM concentration was detected between smokers (5.08 ± 1.72 nmol/mL, 16.01 ± 4.94 ng/mg creatinine, and 5.87 ± 0.98 µm, respectively) and nonsmokers (3.84 ± 0.98 nmol/mL, 13.74 ± 3.60 ng/mg creatinine, and 5.32 ± 0.69 µm, respectively). A similar pattern was obtained for the control population. We did not obtain significant variations for alcoholics and tobacco chewers. A significant increase in all these three biomarkers was observed with the increase in the period of work exposure in tandoor workers. Multivariate regression analysis also revealed that urinary 8-oxodG, MDA, and TM were statistically significantly related to age and period of work exposure. Overall, the present study showed that the exposure to wood smoke in tandoor workers under occupational conditions led to increased DNA damage because of oxidative stress and genotoxicity. These biomarkers, therefore, are good indices to assess oxidative DNA damage in these workers exposed to occupational genotoxicants. It is also necessary to make preventive changes in work conditions and lifestyle, which will help these occupational workers to lead a healthy life.
我们对烤炉工人进行了一项队列研究，以评估氧化损伤和遗传毒性损伤的生物标志物与多环芳烃暴露之间的关系。研究了一系列氧化损伤和遗传毒性损伤的生物标志物，包括尿8-羟基-2'-脱氧鸟苷（8-OHdG）、丙二醛（MDA）含量以及彗星试验中的尾矩（TM）。总共纳入了76名烤炉工人和79名在人口统计学上匹配的健康个体作为对照。我们的结果表明，与对照人群相比，烤炉工人尿中1-羟基芘、尿8-OHdG、MDA含量和TM水平显著更高。在对照人群和烤炉工人中，所有这些生物标志物的浓度均随年龄增加而升高。在烤炉工人中，吸烟者（分别为5.08±1.72 nmol/mL、16.01±4.94 ng/mg肌酐和5.87±0.98 µm）和非吸烟者（分别为3.84±0.98 nmol/mL、13.74±3.60 ng/mg肌酐和5.32±0.69 µm）之间的MDA、8-氧代脱氧鸟苷（8-oxo-2'-deoxyguanosine）和TM浓度存在显著差异。对照人群也得到了类似的结果。对于酗酒者和嚼烟者，我们未观察到显著差异。随着烤炉工人工作暴露时间的增加，所有这三种生物标志物均显著增加。多变量回归分析还显示，尿8-氧代脱氧鸟苷、MDA和TM与年龄和工作暴露时间在统计学上显著相关。总体而言，本研究表明，职业条件下烤炉工人接触木烟会因氧化应激和遗传毒性导致DNA损伤增加。因此，这些生物标志物是评估这些接触职业性遗传毒物的工人氧化DNA损伤的良好指标。对工作条件和生活方式进行预防性改变也很有必要，这将有助于这些职业工人过上健康的生活。
