Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QZ, Great Britain.
Department of Earth Sciences, University of Oxford, South Parks Road, Oxford OX1 3AN, Great Britain.
Anal Chem. 2022 Sep 20;94(37):12664-12672. doi: 10.1021/acs.analchem.2c01971. Epub 2022 Sep 8.
Although, in principle, the Coulter Counter technique yields an absolute measure of particle volume, in practice, calibration is near-universally employed. For regularly shaped and non-biological samples, the use of latex beads for calibration can provide sufficient accuracy. However, this is not the case with particles encased in biogenically formed calcite. To date, there has been no effective route by which a Coulter Counter can be calibrated to enable the calcification of coccolithophores─single cells encrusted with biogenic calcite─to be quantified. Consequently, herein, we seek to answer the following question: - Through the development of a new calibration methodology, based on the measurement and dynamic tracking of the acid-driven calcite dissolution reaction, a route by which the cellular calcite content can be determined is presented. This new method allows, for the first time, a Coulter Counter to be used to yield an absolute measurement of the amount of calcite per cell.
虽然库尔特计数器技术原则上可以提供颗粒体积的绝对测量值，但实际上，几乎普遍都采用了校准。对于形状规则且非生物的样本，使用乳胶珠进行校准可以提供足够的准确性。然而，对于包裹在生物形成的方解石中的颗粒，这种方法并不适用。迄今为止，还没有有效的方法可以校准库尔特计数器，以量化包裹在生物方解石中的颗石藻——一种被生物方解石包裹的单细胞。因此，在这里，我们试图回答以下问题：- 通过开发一种新的校准方法，基于对酸驱动的方解石溶解反应的测量和动态跟踪，提出了一种可以确定细胞中方解石含量的方法。这种新方法首次允许使用库尔特计数器对每个细胞中方解石的含量进行绝对测量。
