González J L, Santo-Benito F F
Brain Res. 1987 May 26;412(1):148-50. doi: 10.1016/0006-8993(87)91450-8.
Choline acetyltransferase (ChAT) activity was measured in a fraction of endothelial cells obtained by collagenase digestion from previously isolated rat brain cortex capillaries. ChAT specific activity in the dissociated cells (0.264 nmol X mg protein-1 X min-1) was nearly 7 times higher than the activity measured in 'capillary-forming' cells (0.038 nmol X mg protein-1 X min-1). It is not known whether this increase was caused by ChAT enrichment of the former cellular fraction, and/or by a change in the specific activity of the enzyme during the isolation procedure, but the finding would be in favor of the intrinsic localization of cerebrovascular ChAT in endothelial cells. This would support the hypothesis of a role for locally synthesized ACh in the vasodilating response to noxious stimuli.
通过胶原酶消化从先前分离的大鼠脑皮质毛细血管中获得的一部分内皮细胞，对胆碱乙酰转移酶（ChAT）活性进行了测定。解离细胞中的ChAT比活性（0.264 nmol·mg蛋白⁻¹·min⁻¹）几乎比“毛细血管形成”细胞中测得的活性（0.038 nmol·mg蛋白⁻¹·min⁻¹）高7倍。尚不清楚这种增加是由前一种细胞组分中ChAT的富集引起的，还是由分离过程中酶的比活性变化引起的，但这一发现支持脑血管ChAT在内皮细胞中的内在定位。这将支持局部合成的乙酰胆碱在对有害刺激的血管舒张反应中起作用的假说。
