大鼠皮质毛细血管胆碱乙酰转移酶活性随衰老而降低。

Decrease of choline acetyltransferase activity of rat cortex capillaries with aging.

作者信息

Santos-Benito F F, Gonzalez J L

出版信息

J Neurochem. 1985 Aug;45(2):633-6. doi: 10.1111/j.1471-4159.1985.tb04034.x.

DOI:10.1111/j.1471-4159.1985.tb04034.x

Abstract

Choline acetyltransferase (ChAT) activity was estimated in brain cortex capillaries isolated from 3-, 12-, 18-, and 24-month-old rats. Maximum enzymatic activity was found at 12 months (55 +/- 0.3 pmol X mg-1 protein X min-1; mean +/- SEM) and then it decreased to reach a minimum at 24 months (34 +/- 3.1 pmol X mg-1 protein X min-1). A less marked decrease of enzymatic activity was also found in cortex homogenate and in a synaptosomal fraction obtained from the same groups of rats. Loss of ChAT of brain capillaries with aging could be related to a general phenomenon of cortical cholinergic deficit in that condition.

摘要

在从3个月、12个月、18个月和24个月大的大鼠中分离出的大脑皮质毛细血管中，对胆碱乙酰转移酶（ChAT）活性进行了评估。在12个月时发现酶活性最高（55±0.3皮摩尔×毫克⁻¹蛋白质×分钟⁻¹；平均值±标准误），然后在24个月时降至最低（34±3.1皮摩尔×毫克⁻¹蛋白质×分钟⁻¹）。在相同组大鼠的皮质匀浆和突触体组分中也发现酶活性有较不明显的下降。随着年龄增长，脑毛细血管中ChAT的丧失可能与该情况下皮质胆碱能缺乏的一般现象有关。