Department of Surgery, Medical Sciences Institute, Campinas University (Unicamp), São Paulo, Brazil.
Oral Pathology Section, Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, Brazil.
J Cosmet Laser Ther. 2022 Nov 17;24(6-8):84-90. doi: 10.1080/14764172.2022.2109677. Epub 2022 Sep 8.
Photobiomodulation therapy (PBMT) has become an adjuvant therapeutic possibility in body remodeling procedures. Given this scenario, this study was proposed with the aim of evaluating the effects of PBMT to Light Emitting Diode (LED) associating the red (630 nm) and infrared (850 nm) wavelengths in the subcutaneous fatty tissue. This controlled study of comparative intervention that evaluated a sample of subcutaneous fatty tissue from women with grade II obesity. The participants received the LED PBMT treatment with associated red and infrared wavelengths sequentially on the left side of the abdomen and the right side was considered as control, with the collection of biological material performed at the time of bariatric surgery. For histological and immunohistochemical evaluation, Caspase 3, Cleaved Caspase 3, CD68+, HSL and adipophilin markers were used. The participants showed positivity in the expression of Caspase 3 and Cleaved Caspase (p < .0001), CD68+ macrophages (p < .0001), HSL (p < .0001) and adipophilin (p < .0013) in the intervention sample when compared to the control. PBMT and LED associating red and infrared wavelengths were able to promote autophagic lipolysis induced by adipocyte cell apoptosis in the subcutaneous tissue of obese individuals.
光生物调节疗法（PBMT）已成为身体重塑程序的辅助治疗可能性。鉴于这种情况，本研究旨在评估 PBMT 对皮下脂肪组织的红（630nm）和红外（850nm）波长的发光二极管（LED）联合治疗的效果。这是一项对照性干预研究，评估了 II 级肥胖女性的皮下脂肪组织样本。参与者接受了 LED PBMT 治疗，左侧腹部联合使用红、红外波长，右侧作为对照，在减肥手术时采集生物材料。为了进行组织学和免疫组织化学评估，使用了 Caspase 3、Cleaved Caspase 3、CD68+、HSL 和 adipophilin 标志物。与对照组相比，干预组的 Caspase 3 和 Cleaved Caspase 表达呈阳性（p<0.0001）、CD68+巨噬细胞（p<0.0001）、HSL（p<0.0001）和 adipophilin（p<0.0013）。PBMT 和 LED 联合使用红、红外波长能够促进肥胖个体皮下组织中脂肪细胞凋亡诱导的自噬性脂肪分解。
