Ren Aigang, Li Qianqian, Guo Yuntong, Cui Xiaolong, Wang Liwei, Huo Yaoliang, Chen Hao, Liu Hui, Huang He
Department of Gastrointestinal Surgery, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China.
Department of Gastroenterology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030013, China.
Transl Oncol. 2022 Nov;25:101506. doi: 10.1016/j.tranon.2022.101506. Epub 2022 Sep 5.
Gastric cancer (GC) is one of the most common cancers worldwide and the majority of GC patients are diagnosed at advanced stages due to the lack of early detection biomarkers. LncRNAs have been shown to play important roles in various diseases and could be predictive biomarkers and therapeutic targets. Our study demonstrated that low expression of lncRNA APTR could promote gastric cancer progression.
Differentiated expressed lncRNAs were identified through analyzing TCGA paired GC RNA sequencing data. LncRNA APTR's clinical relevance was analyzed using the TCGA dataset and GEO datasets. APTR expression in patient samples was detected through qPCR. The proliferation, colony formation, and migration of GC cells were tested. Bioinformatic analyses were performed to explore APTR-affected signaling pathways in GC.
LncRNA APTR is lower expressed in gastric tumor samples and low expression of APTR predicts a poor diagnosis and outcome in GC patients. Silencing APTR promotes gastric cancer proliferation and invasiveness. APTR expression is negatively correlated with inflammatory signaling in the gastric tumor microenvironment.
Our study showed that low expression of lncRNA APTR in gastric cancer is correlated with tumorigenesis and poor diagnosis and prognosis, which is a potential biomarker for gastric cancer patients' diagnosis and treatment.
胃癌（GC）是全球最常见的癌症之一，由于缺乏早期检测生物标志物，大多数胃癌患者在晚期才被诊断出来。长链非编码RNA（lncRNAs）已被证明在各种疾病中发挥重要作用，并且可能是预测性生物标志物和治疗靶点。我们的研究表明lncRNA APTR的低表达可促进胃癌进展。
通过分析TCGA配对的胃癌RNA测序数据来鉴定差异表达的lncRNAs。使用TCGA数据集和GEO数据集分析lncRNA APTR的临床相关性。通过qPCR检测患者样本中APTR的表达。检测胃癌细胞的增殖、集落形成和迁移。进行生物信息学分析以探索APTR在胃癌中影响的信号通路。
lncRNA APTR在胃肿瘤样本中表达较低，APTR的低表达预示着胃癌患者的诊断和预后较差。沉默APTR可促进胃癌增殖和侵袭。APTR表达与胃肿瘤微环境中的炎症信号呈负相关。
我们的研究表明，lncRNA APTR在胃癌中的低表达与肿瘤发生、诊断不良和预后不良相关，这是胃癌患者诊断和治疗的潜在生物标志物。
