Department of Plant Physiology, Faculty of Biology, Adam Mickiewicz University, ul. Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland.
Sustainable Agricultural Systems Laboratory, United States Department of Agriculture, Agricultural Research Service, Henry A. Wallace Beltsville Agricultural Research Center, Beltsville, MD 20705-2350, USA.
Int J Biol Macromol. 2022 Nov 30;221:585-603. doi: 10.1016/j.ijbiomac.2022.09.006. Epub 2022 Sep 6.
We explored the polyamine (PA) metabolic pathway genes in barley (Hv) to understand plant development and stress adaptation in Gramineae crops with emphasis on leaf senescence. Bioinformatics and functional genomics tools were utilized for genome-wide identification, comprehensive gene features, evolution, development and stress effects on the expression of the polyamine metabolic pathway gene families (PMGs). Three S-adenosylmethionine decarboxylases (HvSAMDCs), two ornithine decarboxylases (HvODCs), one arginine decarboxylase (HvADC), one spermidine synthase (HvSPDS), two spermine synthases (HvSPMSs), five copper amine oxidases (HvCuAOs) and seven polyamine oxidases (HvPAOs) members of PMGs were identified and characterized in barley. All the HvPMG genes were found to be distributed on all chromosomes of barley. The phylogenetic and comparative assessment revealed that PA metabolic pathway is highly conserved in plants and the prediction of nine H. vulgare miRNAs (hvu-miR) target sites, 18 protein-protein interactions and 961 putative CREs in the promoter region were discerned. Gene expression of HvSAMDC3, HvCuAO7, HvPAO4 and HvSPMS1 was apparent at every developmental stage. SPDS/SPMS gene family was found to be the most responsive to induced leaf senescence. This study provides a reference for the functional investigation of the molecular mechanism(s) that regulate polyamine metabolism in plants as a tool for future breeding decision management systems.
我们探索了大麦 (Hv) 中的多胺 (PA) 代谢途径基因，以了解禾本科作物的植物发育和应激适应，重点是叶片衰老。生物信息学和功能基因组学工具被用于全基因组鉴定、综合基因特征、进化、发育和应激对多胺代谢途径基因家族 (PMGs) 的表达的影响。三个 S-腺苷甲硫氨酸脱羧酶 (HvSAMDCs)、两个鸟氨酸脱羧酶 (HvODCs)、一个精氨酸脱羧酶 (HvADC)、一个 spermidine synthase (HvSPDS)、两个 spermine synthases (HvSPMSs)、五个铜胺氧化酶 (HvCuAOs) 和七个多胺氧化酶 (HvPAOs) 被鉴定为 PMGs 的成员，并在大麦中进行了特征描述。所有 HvPMG 基因都被发现分布在大麦的所有染色体上。系统发育和比较评估表明，PA 代谢途径在植物中高度保守，预测了 9 个大麦 miRNA (hvu-miR) 靶位点、18 个蛋白质-蛋白质相互作用和启动子区域的 961 个推定 CREs。HvSAMDC3、HvCuAO7、HvPAO4 和 HvSPMS1 的基因表达在每个发育阶段都很明显。SPDS/SPMS 基因家族对诱导的叶片衰老最敏感。这项研究为研究调节植物多胺代谢的分子机制提供了参考，作为未来育种决策管理系统的工具。
