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1
Electron-transport pathway of the NADH-dependent haem oxygenase system of rat liver microsomal fraction induced by cobalt chloride.氯化钴诱导的大鼠肝微粒体部分依赖NADH的血红素加氧酶系统的电子传递途径。
Biochem J. 1979 Feb 15;178(2):323-9. doi: 10.1042/bj1780323.
2
Topological arrangement in microsomal membranes of hepatic haem oxygenase induced by cobalt chloride.氯化钴诱导的肝血红素加氧酶在微粒体膜中的拓扑排列
Biochem J. 1979 Feb 15;178(2):331-7. doi: 10.1042/bj1780331.
3
Evidence for a predominantly NADH-dependent O-dealkylating system in rat hepatic microsomes.大鼠肝微粒体中主要存在依赖于NADH的O-脱烷基化系统的证据。
Biochem Pharmacol. 1985 Dec 15;34(24):4215-28. doi: 10.1016/0006-2952(85)90276-x.
4
Specific requirement of NADPH-cytochrome c reductase for the microsomal heme oxygenase reaction yielding biliverdin IX alpha.NADPH-细胞色素c还原酶对微粒体血红素加氧酶反应生成胆绿素IXα的特殊要求。
FEBS Lett. 1979 Feb 15;98(2):281-4. doi: 10.1016/0014-5793(79)80200-8.
5
Expression of heme oxygenase in hemopoiesis.血红素加氧酶在造血过程中的表达。
Adv Exp Med Biol. 1988;241:97-116. doi: 10.1007/978-1-4684-5571-7_13.
6
Purification and characterization of heme oxygenase from chick liver. Comparison of the avian and mammalian enzymes.鸡肝血红素加氧酶的纯化与特性分析。禽类与哺乳动物酶的比较。
Eur J Biochem. 1990 Apr 20;189(1):155-66. doi: 10.1111/j.1432-1033.1990.tb15472.x.
7
Roles of cytochrome b5 in the oxidation of testosterone and nifedipine by recombinant cytochrome P450 3A4 and by human liver microsomes.细胞色素b5在重组细胞色素P450 3A4和人肝微粒体对睾酮及硝苯地平氧化反应中的作用。
Arch Biochem Biophys. 1996 Jan 15;325(2):174-82. doi: 10.1006/abbi.1996.0022.
8
The catalysis of heme degradation by purified NADPH-cytochrome C reductase in the absence of other microsomal proteins.在不存在其他微粒体蛋白的情况下，纯化的NADPH-细胞色素C还原酶对血红素降解的催化作用。
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Pyridine nucleotide involvement in rat hepatic drug metabolism. The influence of NADH on the NADPH kinetics of phenobarbital pre-treated rat microsomes.吡啶核苷酸与大鼠肝脏药物代谢。NADH对苯巴比妥预处理大鼠微粒体NADPH动力学的影响。
Aust J Exp Biol Med Sci. 1980 Feb;58(1):49-60. doi: 10.1038/icb.1980.5.

引用本文的文献

1
Function and induction of the microsomal heme oxygenase.微粒体血红素加氧酶的功能与诱导
Mol Cell Biochem. 1983;53-54(1-2):163-83. doi: 10.1007/BF00225252.
2
Topological arrangement in microsomal membranes of hepatic haem oxygenase induced by cobalt chloride.氯化钴诱导的肝血红素加氧酶在微粒体膜中的拓扑排列
Biochem J. 1979 Feb 15;178(2):331-7. doi: 10.1042/bj1780331.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The purification and properties of microsomal cytochrome reductase.微粒体细胞色素还原酶的纯化及特性
J Biol Chem. 1957 Oct;228(2):785-99.
3
Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.组织分级分离研究。6. 大鼠肝脏组织中酶的细胞内分布模式。
Biochem J. 1955 Aug;60(4):604-17. doi: 10.1042/bj0600604.
4
The effect of cobalt chloride administration on the synthesis of hepatic microsomal cytochrome P-450.氯化钴给药对肝微粒体细胞色素P-450合成的影响。
Biochem Biophys Res Commun. 1971 Feb 19;42(4):589-95. doi: 10.1016/0006-291x(71)90528-6.
5
The effect of noradrenaline on glyceride synthesis and oxidative metabolism in vitro in the brown fat of newborn rabbits.去甲肾上腺素对新生兔棕色脂肪体外甘油酯合成及氧化代谢的影响。
Biochem J. 1971 Nov;125(1):1-8. doi: 10.1042/bj1250001.
6
Cobalt induction of hepatic heme oxygenase; with evidence that cytochrome P-450 is not essential for this enzyme activity.钴对肝脏血红素加氧酶的诱导作用；有证据表明细胞色素P-450对此酶活性并非必不可少。
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4293-7. doi: 10.1073/pnas.71.11.4293.
7
Effect of cobalt on the synthesis and degradation of hepatic catalase in vivo.钴对体内肝脏过氧化氢酶合成与降解的影响。
Biochem J. 1974 Dec;144(3):455-64. doi: 10.1042/bj1440455.
8
Studies on the microsomal reduced nicotinamide adenine dinucleotide phosphate-cytochrome c reductase from rabbit liver.兔肝微粒体还原型烟酰胺腺嘌呤二核苷酸磷酸 - 细胞色素c还原酶的研究
J Biochem. 1969 Sep;66(3):351-60. doi: 10.1093/oxfordjournals.jbchem.a129153.
9
The enzymatic conversion of heme to bilirubin by microsomal heme oxygenase.微粒体血红素加氧酶将血红素酶促转化为胆红素。
Proc Natl Acad Sci U S A. 1968 Oct;61(2):748-55. doi: 10.1073/pnas.61.2.748.
10
Immunochemical evidence for an association of heme oxygenase with the microsomal electron transport system.血红素加氧酶与微粒体电子传递系统关联的免疫化学证据。
J Biol Chem. 1972 Jun 10;247(11):3601-7.

氯化钴诱导的大鼠肝微粒体部分依赖NADH的血红素加氧酶系统的电子传递途径。

Electron-transport pathway of the NADH-dependent haem oxygenase system of rat liver microsomal fraction induced by cobalt chloride.

作者信息

Hino Y, Minakami S

出版信息

Biochem J. 1979 Feb 15;178(2):323-9. doi: 10.1042/bj1780323.

DOI:10.1042/bj1780323
PMID:36076
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1186518/
Abstract

The hepatic microsomal haem oxygenase activity of rats treated with CoCl2 was studied kinetically by measuring biliverdin, the immediate product of the reaction. Biliverdin was extracted with diethyl ether/ethanol mixture, and was determined by the difference between A690 and A800. The apparent Km value for NADPH (at 50 microM-haematin) was about 0.2 microM when an NADPH-generating system was used, whereas that for NADH was about 630 microM. Essentially the same Vmax. values were obtained for both the NADH- and NADPH-dependent haem oxygenase reactions. No synergism was observed with NADH and NADPH. The NADH-dependent reaction was competitively inhibited by NADP+, with a Ki of about 10 microM. The inhibitoin of the NADH-dependent reaction by the antibody against rat liver microsomal NADPH-cytochrome c reductase was essentially complete, with a pattern similar to that of the NADPH-dependent reaction. The immunochemical experiment and the comparison of the kinetic values with the reported data on isolated NADH-cytochrome b5 reductase and NADPH--cytochrome c reductase indicated the involvement of the latter enzyme in NADH-dependent haem oxygenation by microsomal fraction in situ.

摘要

通过测量反应的直接产物胆绿素，对用氯化钴处理的大鼠肝微粒体血红素加氧酶活性进行了动力学研究。胆绿素用乙醚/乙醇混合物萃取，并通过A690与A800之间的差值进行测定。当使用NADPH生成系统时，NADPH（在50微摩尔血红素存在下）的表观Km值约为0.2微摩尔，而NADH的表观Km值约为630微摩尔。NADH依赖性和NADPH依赖性血红素加氧酶反应获得的Vmax值基本相同。未观察到NADH和NADPH之间的协同作用。NADP +竞争性抑制NADH依赖性反应，Ki约为10微摩尔。抗大鼠肝微粒体NADPH - 细胞色素c还原酶抗体对NADH依赖性反应的抑制基本完全，其模式与NADPH依赖性反应相似。免疫化学实验以及动力学值与关于分离的NADH - 细胞色素b5还原酶和NADPH - 细胞色素c还原酶的报道数据的比较表明，后一种酶参与了微粒体部分原位NADH依赖性血红素加氧反应。