去甲肾上腺素对新生兔棕色脂肪体外甘油酯合成及氧化代谢的影响。
The effect of noradrenaline on glyceride synthesis and oxidative metabolism in vitro in the brown fat of newborn rabbits.
作者信息
Knight B L, Myant N B
出版信息
Biochem J. 1971 Nov;125(1):1-8. doi: 10.1042/bj1250001.
Abstract
- The effect of noradrenaline on the synthesis of glyceride from [U-(14)C]glucose and on gas exchange in the brown fat of newborn rabbits in vitro was investigated. 2. The specific radioactivity of l-glycerol 3-phosphate was lower than that of lactate, presumably because glycerol derived from glyceride was rephosphorylated by glycerokinase. 3. In the basal state more than 25% of the total respiration was due to pyruvate oxidation. Noradrenaline stimulated glyceride synthesis and total respiration without changing the proportion of the total respiration due to pyruvate oxidation. 4. The extra ADP released by noradrenaline stimulation of glyceride synthesis could not have supported more than 2% of the observed increase in substrate oxidation if mitochondria from brown-fat-cells remain fully coupled in the stimulated state, but could have supported about one-third of the observed increase if they become uncoupled in the presence of noradrenaline.
摘要
- 研究了去甲肾上腺素对新生兔棕色脂肪体外由[U-(14)C]葡萄糖合成甘油酯及气体交换的影响。2. L-3-磷酸甘油的比放射性低于乳酸，推测是因为甘油酯衍生的甘油被甘油激酶重新磷酸化。3. 在基础状态下，总呼吸的25%以上归因于丙酮酸氧化。去甲肾上腺素刺激甘油酯合成和总呼吸，而不改变丙酮酸氧化在总呼吸中所占的比例。4. 如果棕色脂肪细胞的线粒体在受刺激状态下仍保持完全偶联，去甲肾上腺素刺激甘油酯合成释放的额外ADP不可能支持超过观察到的底物氧化增加量的2%，但如果它们在去甲肾上腺素存在下变得解偶联，则可能支持约三分之一观察到的增加量。
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