College of Food Science and Technology, International Joint Laboratory of Meat Processing and Safety in Henan province, Henan Agricultural University, No.63 Wenhua Rd, Zhengzhou 450002, PR China; International Joint Laboratory of Meat Processing and Safety in Henan province, Henan Agricultural University, No.63 Wenhua Rd, Zhengzhou 450002, PR China.
College of Food Science and Technology, International Joint Laboratory of Meat Processing and Safety in Henan province, Henan Agricultural University, No.63 Wenhua Rd, Zhengzhou 450002, PR China; International Joint Laboratory of Meat Processing and Safety in Henan province, Henan Agricultural University, No.63 Wenhua Rd, Zhengzhou 450002, PR China.
Food Res Int. 2022 Oct;160:111426. doi: 10.1016/j.foodres.2022.111426. Epub 2022 May 27.
In this study, we developed a novel and sensitive AgNPs self-assembled solid-phase (AgNPs-SASP) SERS substrate platform using seed-mediated and liquid interface self-assembly methods and applied the platform for the detection of three bacterial spores. Multivariate statistical analysis (HCA and LDA) were employed for identification and categorization of the obtained data. The results illustrated that AgNPs-SASP exhibited high reproducibility and Raman enhancement effect (1.43 × 10). The Raman shift bands of the three bacterial spores ranged from 400 to 1800 cm demonstrating the difference in reigon and intensity. And the intensities of their Ca-DPA Raman bands are significantly different. HCA results revealed that the spectra for the three bacterial spores were statistically different while LDA completely differentiated the spectra for the three bacterial spores with 100 % sensitivity and specificity. Overall, the novel SERS platform based on AgNPs-SASP provides an effective tool for food safety risk control and detection.
在这项研究中，我们使用种子介导和液-固界面自组装方法开发了一种新型灵敏的 AgNPs 自组装固相（AgNPs-SASP）SERS 基底平台，并将该平台用于三种细菌孢子的检测。采用多元统计分析（HCA 和 LDA）对获得的数据进行识别和分类。结果表明，AgNPs-SASP 表现出高重现性和拉曼增强效应（1.43×10）。三种细菌孢子的拉曼位移带范围从 400 到 1800cm，表明区域和强度存在差异。它们的 Ca-DPA 拉曼带的强度也有显著差异。HCA 结果表明，三种细菌孢子的光谱在统计学上存在差异，而 LDA 则完全区分了三种细菌孢子的光谱，具有 100%的灵敏度和特异性。总的来说，基于 AgNPs-SASP 的新型 SERS 平台为食品安全风险控制和检测提供了一种有效的工具。
