Tian Jiaqi, Tu Qiancheng, Li Miaoyun, Zhao Lijun, Zhu Yaodi, Lee Jong-Hoon, Gai Zhengyan, Zhao Gaiming, Ma Yangyang
College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.
International Joint Laboratory of Meat Processing and Safety in Henan Province, Henan Agricultural University, Zhengzhou 450002, PR China.
Food Chem X. 2023 Dec 14;21:101069. doi: 10.1016/j.fochx.2023.101069. eCollection 2024 Mar 30.
Foodborne spores are ubiquitous with extremely strong resistance, and pose a serious threat to food safety and human health. Therefore, rapid, sensitive, and selective detection of spores are crucial. In this study, a fluorescent probe was developed based on lanthanide ion (Eu)-labeled nano-silver-modified graphene oxide (GO-AgNPs-Eu) for the detection of 2,6-dipicolinic acid (DPA), a biomarker unique to spores, to allow quantitative spores detection. The GO-AgNPs-Eu nano-fluorescent probe was loaded onto a polyvinylidene fluoride microfiltration membrane, and a smartphone-assisted portable GO-AgNPs-Eu nanoparticles-based paper visual sensor was designed for rapid on-site quantitative and real-time online detection of spores. The results indicated that the developed probe achieved equilibrium binding with DPA within 5 min, and enhanced fluorescence emission through antenna effect. The fluorescence detection presented a good linear relationship in the DPA concentration range of 0-45 μM, with a DPA detection limit of 4.62 nM and spore detection limit of 10 cfu/mL. The developed sensor showed a change in fluorescence from blue to red with increasing DPA concentration, and this color change was quantitatively detected through smartphone RGB variations, with a detection limit of 13.1 μM for DPA and 6.3 cfu/mL for spores. Subsequently, the sensitivity and selectivity of the developed sensor were verified using actual milk and water samples spiked with spores. The results of this study provided objective technological support for rapid detection of spores, which is important for reducing the occurrence of foodborne diseases and improving food safety.
食源性病原体芽孢无处不在，具有极强的抗性，对食品安全和人类健康构成严重威胁。因此，快速、灵敏且选择性地检测芽孢至关重要。在本研究中，基于镧系离子（铕）标记的纳米银修饰氧化石墨烯（GO-AgNPs-Eu）开发了一种荧光探针，用于检测芽孢特有的生物标志物2,6-吡啶二甲酸（DPA），以实现芽孢的定量检测。将GO-AgNPs-Eu纳米荧光探针负载到聚偏二氟乙烯微滤膜上，设计了一种基于智能手机辅助的便携式GO-AgNPs-Eu纳米颗粒纸质视觉传感器，用于芽孢的快速现场定量和实时在线检测。结果表明，所开发的探针在5分钟内与DPA达到平衡结合，并通过天线效应增强荧光发射。荧光检测在0-45μM的DPA浓度范围内呈现良好的线性关系，DPA检测限为4.62 nM，芽孢检测限为10 cfu/mL。所开发的传感器随着DPA浓度的增加，荧光颜色从蓝色变为红色，并且通过智能手机RGB变化对这种颜色变化进行定量检测，DPA检测限为13.1μM，芽孢检测限为6.3 cfu/mL。随后，使用添加了芽孢的实际牛奶和水样验证了所开发传感器的灵敏度和选择性。本研究结果为芽孢的快速检测提供了客观的技术支持，这对于减少食源性疾病的发生和提高食品安全具有重要意义。
