选定类黄酮对用于培养肉生产的猪肌肉干细胞增殖和分化的影响。
Effects of selected flavonoids oncellproliferation and differentiation of porcine muscle stem cells for cultured meat production.
机构信息
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; National Center of Meat Quality and Safety Control, Key Laboratory of Meat Processing and Quality Control, Key Laboratory of Meat Processing, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
出版信息
Food Res Int. 2022 Oct;160:111459. doi: 10.1016/j.foodres.2022.111459. Epub 2022 Jun 4.
Stemness decline of muscle stem cells (MuSCs) is a significant problem in cultured meat processing. In the present study, three flavonoids (quercetin, icariin, and 3,2'-dihydroxyflavone) with multi concentrations were evaluated to promote the proliferation and differentiation of porcine muscle stem cells. In the proliferation phase, 3,2'-dihydroxyflavone (10 μM) significantly amplified the cells by 34% and up-regulated the expression of paired box transcription factor 7 (PAX7) by 60%, which was higher than quercetin (75 nM) and icariin (7.5 nM). In the differentiation phase, quercetin (50 nM) showed the best pro-differentiation effect and up-regulated the expression of myosin heavy chain (MYHC) by 4.73-fold compared with the control group. These results indicated that flavonoids had a significant impact on promoting the proliferation and differentiation of porcine MuSCs, and 3,2'-dihydroxyflavone (10 μM) for proliferation and quercetin (50 nM) for differentiation were the optimal combinations.
肌肉干细胞（MuSCs）的干性衰退是培养肉加工中的一个重大问题。在本研究中，评估了三种黄酮类化合物（槲皮素、淫羊藿苷和 3,2'-二羟基黄酮）的多个浓度，以促进猪肌肉干细胞的增殖和分化。在增殖阶段，3,2'-二羟基黄酮（10 μM）将细胞显著扩增了 34%，并将配对盒转录因子 7（PAX7）的表达上调了 60%，高于槲皮素（75 nM）和淫羊藿苷（7.5 nM）。在分化阶段，与对照组相比，槲皮素（50 nM）表现出最佳的促分化效果，将肌球蛋白重链（MYHC）的表达上调了 4.73 倍。这些结果表明，黄酮类化合物对促进猪 MuSCs 的增殖和分化具有显著影响，其中 3,2'-二羟基黄酮（10 μM）促进增殖，槲皮素（50 nM）促进分化是最佳组合。
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