School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Republic of Korea.
Int J Mol Sci. 2024 Jul 24;25(15):8059. doi: 10.3390/ijms25158059.
, particularly drug-resistant strains, poses significant challenges in healthcare due to its ability to form biofilms, which confer increased resistance to antibiotics and immune responses. Building on previous knowledge that several flavonoids exhibit antibiofilm activity, this study sought to identify a novel flavonoid capable of effectively inhibiting biofilm formation and virulence factor production in strains including MRSA. Among the 19 flavonoid-like compounds tested, 3,2'-dihydroxyflavone (3,2'-DHF) was identified for the first time as inhibiting biofilm formation and virulence factors in with an MIC 75 µg/mL. The antibiofilm activity was further confirmed by microscopic methods. Notably, 3,2'-DHF at 5 µg/mL was effective in inhibiting both mono- and polymicrobial biofilms involving and , a common co-pathogen. 3,2'-DHF reduces hemolytic activity, slime production, and the expression of key virulence factors such as hemolysin gene and nuclease gene in . These findings highlight the potential of 3,2'-DHF as a novel antibiofilm and antivirulence agent against both bacterial and fungal biofilms, offering a promising alternative to traditional antibiotics in the treatment of biofilm-associated infections.
, 特别是耐药菌株，由于其形成生物膜的能力，对医疗保健构成了重大挑战，生物膜增加了对抗生素和免疫反应的抵抗力。基于先前的知识，即几种类黄酮具有抗生物膜活性，本研究旨在确定一种新型类黄酮，能够有效抑制包括 MRSA 在内的 菌株的生物膜形成和毒力因子产生。在测试的 19 种类黄酮样化合物中，3,2'-二羟基黄酮（3,2'-DHF）首次被鉴定为能够抑制 生物膜形成和毒力因子，MIC75μg/mL。通过显微镜方法进一步证实了抗生物膜活性。值得注意的是，3,2'-DHF 在 5μg/mL 时有效抑制涉及 和 的单种和混合生物膜， 是一种常见的共病原体。3,2'-DHF 降低了溶血活性、粘液产生以及溶血素基因和核酸酶基因等关键毒力因子的表达 在 中。这些发现强调了 3,2'-DHF 作为一种新型抗生物膜和抗毒力剂的潜力，可对抗细菌和真菌生物膜，为治疗生物膜相关感染提供了一种有前途的替代传统抗生素的方法。
