Wang Binbin, Huo Lele, Tian Wanru, Xing Wenwen, Luo Gang, Li Mengxuan, Sun Liumei, Liu Jiying
Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.
Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang, 212100, China.
Vet Res Commun. 2025 Aug 15;49(5):281. doi: 10.1007/s11259-025-10858-x.
The gastrointestinal health of livestock is essential for the agricultural sector. Rutin, a natural flavonoid extract from plants, exhibits antioxidant and anti-inflammatory properties, alongside a protective impact on the intestinal tract of pigs. However its influence on the proliferation, migration, and cellular junctions of IPEC-J2 cells remains unexplored. This study involved treating IPEC-J2 cells with varying concentrations of rutin. It was observed that after 24 h of treatment with 200 µM rutin, cell viability was significantly increased, along with significant upregulation in the mRNA expression levels of proliferation-related genes (CCNE2, CDK4, CDK2, CCNDB, and PCNA) and tight junction genes (ZO-1, Occludin, and Claudin-1). Additionally, the expression of proliferation-related PCNA protein was significantly increased. Transcriptome sequencing revealed that rutin treatment impacted the overall transcription profile of IPEC-J2 cells, altering the expression of genes involved in critical physiological processes such as redox balance, cell proliferation, and tight junctions maintenance. Consequently, rutin demonstrated a significant enhancement in the proliferation of IPEC-J2 cells and improved the intestinal barrier function in pigs, offering valuable insights for sustaining pig intestinal health in swine husbandry.
家畜的胃肠道健康对农业部门至关重要。芦丁是一种从植物中提取的天然类黄酮，具有抗氧化和抗炎特性，对猪的肠道也有保护作用。然而，其对IPEC-J2细胞增殖、迁移和细胞连接的影响仍未得到探索。本研究用不同浓度的芦丁处理IPEC-J2细胞。结果发现，用200µM芦丁处理24小时后，细胞活力显著增加，增殖相关基因（CCNE2、CDK4、CDK2、CCNDB和PCNA）和紧密连接基因（ZO-1、闭合蛋白和Claudin-1）的mRNA表达水平也显著上调。此外，增殖相关的PCNA蛋白表达也显著增加。转录组测序显示，芦丁处理影响了IPEC-J2细胞的整体转录谱，改变了参与氧化还原平衡、细胞增殖和紧密连接维持等关键生理过程的基因表达。因此，芦丁显著促进了IPEC-J2细胞的增殖，改善了猪的肠道屏障功能，为养猪业维持猪的肠道健康提供了有价值的见解。
