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螺旋管 UV-C 系统对全脂牛奶和杏仁奶中蜡样芽胞杆菌孢子的灭活:流场数值模拟、脂质过氧化和挥发性分析。

Inactivation of B. cereus spores in whole milk and almond milk by serpentine path coiled tube UV-C system: Numerical simulation of flow field, lipid peroxidation and volatiles analysis.

机构信息

Food Biosciences and Technology Program, Department of Agricultural and Environmental Sciences, Tennessee State University, Nashville, 37209 TN, USA.

Food Biosciences and Technology Program, Department of Agricultural and Environmental Sciences, Tennessee State University, Nashville, 37209 TN, USA.

出版信息

Food Res Int. 2022 Oct;160:111652. doi: 10.1016/j.foodres.2022.111652. Epub 2022 Jul 8.

DOI:10.1016/j.foodres.2022.111652
PMID:36076441
Abstract

A novel continuous thin-film (1.59 mm) serpentine path coiled tube (SPCT) UV system operating at 254 nm wavelength was designed and compared with flow field distribution of whole milk with helical path coiled tube (HPCT) UV system using computational fluid dynamics. The results revealed efficient velocity magnitude distribution at serpentine bend geometric locations of the SPCT UV system. Further in this study, we evaluatedBacillus cereusSpores inactivation in whole milk (WM) and almond milk (AM) using the developed SPCT UV system. Experimental data showed that > 4 log reduction of spores was achieved after six and ten passes of WM and AM at a flow rate of 70 and 162 mL/min, respectively. The bio-dosimetry method was used to verify the delivered reduction equivalent fluence (REF) and reported as 33 ± 0.73 and 36.5 ± 1.9 mJ/cm. We noticed no significant effect on lipid oxidation and volatiles profile (p > 0.05) up to delivered REF of 60 mJ/cm. This study demonstrated that high levels of inactivation ofB. cereusspores could be feasible with minimal impact on product quality by UV-C processing of dairy and non-dairy opaque scattering fluids.

摘要

设计了一种新型连续薄膜(1.59 毫米)蛇形路径盘管(SPCT)UV 系统,工作波长为 254nm,并使用计算流体动力学与螺旋路径盘管(HPCT)UV 系统对全脂牛奶的流场分布进行了比较。结果表明,SPCT UV 系统的蛇形弯曲几何位置具有高效的速度大小分布。在本研究中,我们使用开发的 SPCT UV 系统评估了全脂牛奶(WM)和杏仁奶(AM)中蜡样芽孢杆菌孢子的失活动力学。实验数据表明,WM 和 AM 在 70 和 162 mL/min 的流速下分别经过六次和十次通过后,孢子减少了>4 个对数。使用生物剂量测定法验证了传递的等效剂量(REF),并报告为 33 ± 0.73 和 36.5 ± 1.9 mJ/cm。我们注意到,在传递 REF 达到 60 mJ/cm 之前,对脂质氧化和挥发性物质分布没有明显影响(p > 0.05)。本研究表明,通过对乳制品和非乳制品不透明散射流体进行 UV-C 处理,可以实现高水平的蜡样芽孢杆菌孢子失活动力学,对产品质量的影响最小。

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