Laboratory of Veterinary Surgery, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan; Department of Veterinary Surgery, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Department of Cytology and Histology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Biomed Pharmacother. 2022 Sep;153:113422. doi: 10.1016/j.biopha.2022.113422. Epub 2022 Jul 19.
Interstitial cystitis (IC) is an incurable chronic disease. The etiology of IC is unclear, and no effective therapies have been established. Here, using a hydrogen chloride (HCL)-induced IC in a rat model, the therapeutic potency of stromal vascular fraction (SVF) and Adipose-derived stem cells (ADSCs) was studied. Thirty-six female Sprague Dawley rats were divided into four groups: the sham, HCL, (HCL+SVF) group, and (HCL+ADSCs) group (9 for each). Cystitis was induced by transurethral instillation of HCL, while PBS was used for the sham group. A single dose of SVF or ADSCs was injected into the submucosa of the rat bladder in HCL-induced IC groups. The bladder tissues were analyzed for Toluidine Blue, Masson Trichrome, CD3, and CD34 to evaluate mast cell activation, fibrosis, inflammatory cells, and bladder regeneration, respectively. Compared to HCL-induced IC, SVF or ADSCs injection into IC bladder dramatically decreased mast cell infiltration, T-cell activation, and fibrosis. Taken together, administration of SVF cells or cultured ADSCs improves the histopathological outcomes of HCL-induced bladder injury in a time-dependent manner. Of note, SVF injection into the bladder submucosa was estimated to have the most potent therapeutic efficacy and may represent an essential component in future clinical applications.
间质性膀胱炎（IC）是一种无法治愈的慢性疾病。IC 的病因尚不清楚，也没有确立有效的治疗方法。在这里，我们使用盐酸（HCL）诱导的大鼠 IC 模型，研究了基质血管部分（SVF）和脂肪来源的干细胞（ADSCs）的治疗效果。36 只雌性 Sprague Dawley 大鼠分为四组：假手术组、HCL 组、（HCL+SVF）组和（HCL+ADSCs）组（每组 9 只）。通过经尿道滴注 HCL 诱导膀胱炎，而假手术组则使用 PBS。在 HCL 诱导的 IC 组中，将单次剂量的 SVF 或 ADSCs 注入大鼠膀胱的黏膜下层。用甲苯胺蓝、马松三色、CD3 和 CD34 分析膀胱组织，分别评估肥大细胞活化、纤维化、炎症细胞和膀胱再生。与 HCL 诱导的 IC 相比，SVF 或 ADSCs 注射到 IC 膀胱中可显著减少肥大细胞浸润、T 细胞活化和纤维化。总之，SVF 细胞或培养的 ADSCs 的给药可在时间依赖性方式改善 HCL 诱导的膀胱损伤的组织病理学结果。值得注意的是，SVF 注射到膀胱黏膜下层的治疗效果最强，可能是未来临床应用的重要组成部分。
