State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, China.
Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA.
Int J Mol Sci. 2022 Aug 26;23(17):9662. doi: 10.3390/ijms23179662.
Wheat stripe (yellow) rust is a worldwide disease that seriously reduces wheat grain yield and quality. Adult-plant resistance (APR) to stripe rust is generally more durable but usually controlled by multiple genes with partial resistance. In this study, a recombinant inbred line population was developed from a cross between a Chinese wheat landrace, Tutoumai, with APR to stripe rust, and a highly susceptible wheat cultivar, Siyang 936. The population was genotyped by genotyping-by-sequencing and phenotyped for APR to stripe rust in four consecutive field experiments. Three QTLs, , , and , were identified for APR to stripe rust, and explained 8.0-21.2%, 10.1-22.7%, and 11.6-18.0% of the phenotypic variation, respectively. was further mapped to a 21.6 Mb region using KASP markers derived from SNPs identified by RNA-seq of the two parents. In the region, 13 disease-resistance-related genes were differently expressed between the two parents, and therefore were considered as the putative candidates of . This study provides favorable gene/QTL and high-throughput markers to breeding programs for marker-assisted selection of the wheat stripe rust APR genes.
小麦条锈病是一种世界性病害，严重降低小麦的籽粒产量和品质。成株期抗性（APR）通常更持久，但通常由多个部分抗性基因控制。本研究利用一个对条锈病具有 APR 的中国小麦地方品种图们和一个高度感病的小麦品种泗阳 936 构建了重组自交系群体。该群体通过测序分型进行基因型分型，并在四个连续的田间试验中对 APR 进行表型鉴定。鉴定到 3 个与 APR 相关的 QTL，分别命名为 、 和 ，解释了 8.0-21.2%、10.1-22.7%和 11.6-18.0%的表型变异。利用从亲本 RNA-seq 鉴定的 SNP 开发的 KASP 标记，将 进一步定位到一个 21.6 Mb 的区域。在 区域内，两个亲本之间有 13 个与疾病抗性相关的基因表达不同，因此被认为是 的候选基因。本研究为小麦条锈病 APR 基因的标记辅助选择提供了有利的基因/QTL 和高通量标记。
