Institute of Animal Sciences, Departments of Animal Breeding, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland.
Int J Mol Sci. 2022 Aug 29;23(17):9771. doi: 10.3390/ijms23179771.
Enzymatic diagnostics have practical applications in diseases of the liver, heart, pancreas, muscles, blood, and neoplastic diseases. This study aimed to compare enzyme activity to describe dairy cows' metabolism during early lactation. Based on their general health symptoms, the cows were assigned to one of three groups: acidotic, healthy and ketotic. Samples of milk, blood and rumen fluid were collected at 12 ± 5 days postpartum. Ketotic cows were characterized by the highest malondialdehyde (MDA, 76.098 nM/mL), glutathione reductase (GluRed, 109.852 U/L), superoxide dismutase (SOD, 294.22 U/L) and gamma-glutamyltranspeptidase (GGTP, 71.175 U/L) activity. In comparing ketotic and acidotic cows, MDA, GluRed, SOD and GGTP activity were higher by a factor of almost: 1.85, 1.89, 0.79 and 2.50, respectively. Acidotic cows were characterized by the highest aspartate aminotransferase activity (AspAT, 125.914 U/L). In comparing acidotic and ketotic cows, AspAT activity was higher by a factor of almost 1.90. The use of enzymatic markers could limit the frequency of sampling for laboratory analyses and may result in a faster diagnosis of metabolic disorders. AspAT activity in blood serum seems to be a good indicator of acidosis; GGTP may participate in the pathogenesis of ketosis.
酶学诊断在肝脏、心脏、胰腺、肌肉、血液和肿瘤疾病中有实际应用。本研究旨在比较酶活性，以描述奶牛在泌乳早期的代谢情况。根据其一般健康症状，将奶牛分为酸中毒、健康和酮病三组。在产后 12±5 天采集奶样、血样和瘤胃液样。酮病奶牛的丙二醛（MDA，76.098nM/mL）、谷胱甘肽还原酶（GluRed，109.852U/L）、超氧化物歧化酶（SOD，294.22U/L）和γ-谷氨酰转肽酶（GGTP，71.175U/L）活性最高。与酮病奶牛相比，酸中毒奶牛的 MDA、GluRed、SOD 和 GGTP 活性分别高出近 1.85、1.89、0.79 和 2.50 倍。酮病奶牛的天冬氨酸氨基转移酶（AspAT，125.914U/L）活性最高。与酮病奶牛相比，酸中毒奶牛的 AspAT 活性高出近 1.90 倍。酶学标志物的使用可以减少实验室分析的采样频率，并可能导致代谢紊乱的快速诊断。血清中 AspAT 活性似乎是酸中毒的良好指标；GGTP 可能参与了酮病的发病机制。
