Department of Anesthesiology, University of Groningen, University Medical Centre Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
Department of Surgery, Amphia Hospital, Molengracht 21, 4818 CK Breda, The Netherlands.
Int J Mol Sci. 2022 Aug 29;23(17):9789. doi: 10.3390/ijms23179789.
During ischemia−reperfusion injury (IRI), reactive oxygen species are produced that can be scavenged by free sulfhydryl groups (R-SH, free thiols). In this study, we hypothesized that R-SH levels decrease as a consequence of renal IRI and that R-SH levels reflect post-transplant graft function. Systemic venous, arterial, renal venous, and urinary samples were collected in donors and recipients before, during, and after transplantation. R-SH was measured colorimetrically. Systemic arterial R-SH levels in recipients increased significantly up to 30 sec after reperfusion (p < 0.001). In contrast, renal venous R-SH levels significantly decreased at 5 and 10 min compared to 30 sec after reperfusion (both p < 0.001). This resulted in a significant decrease in delta R-SH (defined as the difference between renal venous and systemic arterial R-SH levels) till 30 sec after reperfusion (p < 0.001), indicating a net decrease in R-SH levels across the transplanted kidney. Overall, these results suggest trans-renal oxidative stress as a consequence of IRI during kidney transplantation, reflected by systemic and renal changes in R-SH levels in transplant recipients.
在缺血再灌注损伤（IRI）期间，会产生活性氧，这些活性氧可以被游离巯基（R-SH，游离硫醇）清除。在这项研究中，我们假设 R-SH 水平会因肾 IRI 而降低，并且 R-SH 水平反映移植后移植物的功能。在移植前、移植期间和移植后，从供体和受者中采集静脉、动脉、肾静脉和尿液样本。通过比色法测量 R-SH。受者的系统动脉 R-SH 水平在再灌注后 30 秒内显著增加（p < 0.001）。相比之下，肾静脉 R-SH 水平在再灌注后 5 分钟和 10 分钟与 30 秒时相比显著降低（两者均 p < 0.001）。这导致再灌注后 30 秒内 delta R-SH（定义为肾静脉和系统动脉 R-SH 水平之间的差异）显著降低（p < 0.001），表明穿过移植肾的 R-SH 水平净减少，提示肾移植过程中 IRI 导致跨肾氧化应激，这反映在受者的系统和肾 R-SH 水平变化上。
