Analytical Chemistry Department, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, 4 Louis Pasteur Str., 400349 Cluj-Napoca, Romania.
Microbiology Department, Faculty of Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, 4 Louis Pasteur Str., 400349 Cluj-Napoca, Romania.
Int J Mol Sci. 2022 Aug 31;23(17):9884. doi: 10.3390/ijms23179884.
The rapid and decentralized detection of bacteria from biomedical, environmental, and food samples has the capacity to improve the conventional protocols and to change a predictable outcome. Identifying new markers and analysis methods represents an attractive strategy for the indirect but simpler and safer detection of pathogens that could replace existing methods. Enterobactin (Ent), a siderophore produced by or other Gram-negative bacteria, was studied on different electrode materials to reveal its electrochemical fingerprint-very useful information towards the detection of the bacteria based on this analyte. The molecule was successfully identified in culture media samples and a future goal is the development of a rapid antibiogram. The presence of Ent was also assessed in wastewater and treated water samples collected from the municipal sewage treatment plant, groundwater, and tap water. Moreover, a custom configuration printed on a medical glove was employed to detect the target in the presence of another bacterial marker, namely pyocyanin (PyoC), that being a metabolite specific of another pathogen bacterium, namely . Such new mobile and wearable platforms offer considerable promise for rapid low-cost on-site screening of bacterial contamination.
从生物医学、环境和食品样本中快速分散式地检测细菌，有能力改善传统方法并改变可预测的结果。识别新的标记物和分析方法是一种很有吸引力的策略，可以间接但更简单、更安全地检测病原体，从而替代现有方法。肠杆菌素（Ent）是一种由革兰氏阴性菌或其他细菌产生的铁载体，已在不同的电极材料上进行了研究，以揭示其电化学指纹，这对于基于该分析物检测细菌非常有用。该分子已成功在培养基样本中被识别，未来的目标是开发快速抗生素分析。此外，还评估了污水和从市政污水处理厂、地下水和自来水中采集的处理水样中 Ent 的存在情况。此外，使用定制配置在医用手套上进行检测，以检测目标的存在，同时还存在另一种细菌标记物，即绿脓菌素（PyoC），它是另一种病原体细菌，即铜绿假单胞菌的代谢产物。这种新的移动和可穿戴平台为快速低成本的现场细菌污染筛查提供了很大的希望。
