State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
Int J Mol Sci. 2022 Sep 1;23(17):9973. doi: 10.3390/ijms23179973.
Fruit shape, an important agronomic trait of cucumber ( L.), is tightly controlled by a series of genes such as , a homologue of that is responsible for the tomato () fruit shape via the modulation of cell division. However, the direct genetic evidence about the -mediated regulation of fruit shape is still scarce, limiting our mechanistic understanding of the biological functions of . Here, we introduced into the round-fruited tomato inbred line 'SN1' (wild type, WT) via the -mediated method. The high and constitutive expression of was revealed by real-time PCR in all the tested tissues of the transgenic plants, especially in the fruits and ovaries. Phenotypic analyses showed that the ectopic expression of increased fruit length while it decreased fruit diameter, thus leading to the enhanced fruit shape index in the transgenic tomato lines relative to the WT. Additionally, the reduction in the seed size and seed-setting rate and the stimulation of seed germination were observed in the -expressed tomato. A histological survey demonstrated that the elongated fruits were mainly derived from the significant increasing of the longitudinal cell number, which compensated for the negative effects of decreased cell area in the central columellae. These observations are different from action mode of , thus shedding new insights into the -mediated regulation of fruit shape.
果实形状是黄瓜（L.）的一个重要农艺性状，受一系列基因的紧密调控，如，它是番茄（）果实形状的同源物，通过调节细胞分裂对果实形状起作用。然而，关于 - 介导的果实形状调控的直接遗传证据仍然很少，限制了我们对 的生物学功能的机制理解。在这里，我们通过 - 介导的方法将 导入圆形果实的番茄自交系 'SN1'（野生型，WT）中。实时 PCR 分析显示，在所有测试的转基因植物组织中，尤其是在果实和子房中， 的高表达和组成型表达。表型分析表明， 的异位表达增加了果实长度，同时降低了果实直径，从而使转基因番茄系的果实形状指数相对于 WT 增强。此外，在表达 的番茄中观察到种子大小和结实率降低以及种子萌发刺激。组织学研究表明，伸长的果实主要来源于纵向细胞数量的显著增加，这补偿了中央柱状区细胞面积减少的负面影响。这些观察结果与 的作用模式不同，因此为 - 介导的果实形状调控提供了新的见解。
