State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture, College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
Int J Mol Sci. 2022 May 23;23(10):5843. doi: 10.3390/ijms23105843.
Cucumber ( L.) is an economically important vegetable crop with the unique growth habit and typical trailing shoot architecture of Cucurbitaceae. Elucidating the regulatory mechanisms of growth and development is significant for improving quality and productivity in cucumber. Here we isolated a spontaneous cucumber mutant () with multiple morphological changes including root, plant stature, stem, leaf, male and female flowers, as well as fruit. Anatomical and cytological analyses demonstrated that both cell size and number decreased, and the shoot apical meristem (SAM) was smaller in compared with WT. Pollen vigor and germination assays and cross tests revealed that is female sterile, which may be caused by the absence of ovules. Genetic analysis showed that is a recessive single gene mutant. Using the MutMap strategy, the gene was found to be located on chromosome 5. Integrated profiling of transcriptome and proteome indicated that the different expression genes related to hormones and SAM maintenance might be the reason for the phenotypic changes of . These results expanded the insight into the molecular regulation of organ growth and development and provided a comprehensive reference map for further studies in cucumber.
黄瓜（L.）是一种经济上重要的蔬菜作物，具有独特的生长习性和典型的葫芦科蔓生枝结构。阐明生长发育的调控机制对于提高黄瓜的品质和产量具有重要意义。在这里，我们分离到一个自发的黄瓜突变体（），它具有多种形态变化，包括根、植株、茎、叶、雄花和雌花以及果实。解剖学和细胞学分析表明，与 WT 相比，细胞大小和数量均减少，并且茎尖分生组织（SAM）更小。花粉活力和萌发测定和杂交试验表明，是雌性不育的，这可能是由于没有胚珠。遗传分析表明，是一个隐性单基因突变体。利用 MutMap 策略，发现基因位于 5 号染色体上。转录组和蛋白质组的综合分析表明，与激素和 SAM 维持相关的不同表达基因可能是表型变化的原因。这些结果扩展了对器官生长和发育的分子调控的认识，并为进一步研究黄瓜提供了全面的参考图谱。
