National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China.
Int J Mol Sci. 2022 Sep 2;23(17):10057. doi: 10.3390/ijms231710057.
SWEETs (sugars will eventually be exported transporters), a well-known class of sugar transporters, are involved in plant growth and development, sugar transport, biotic and abiotic stresses, etc. However, to date, there have been few investigations of in Orchidaceae. In this study, 23 genes were identified in for the first time, with an MtN3/saliva conserved domain, and were divided into four subgroups by phylogenetic tree. The same subfamily members had similar gene structures and motifs. Multiple -elements related to sugar and environmental stresses were found in the promoter region. Further, 21 genes were localized on 11 chromosomes and 2 paralogous pairs were found via intraspecific collinearity analysis. Expression profiling results showed that were tissue-specific. It also revealed that and were responsive to low temperature and oxidative stresses. In addition, subcellular localization study indicated that and were localized in the cell membrane. This study provided important clues for the in-depth elucidation of the sugar transport mechanism of genes and their functional roles in response to abiotic stresses.
SWEETs（糖最终会被输出的转运蛋白）是一类众所周知的糖转运蛋白，参与植物的生长发育、糖转运、生物和非生物胁迫等过程。然而，迄今为止，对兰科植物中的研究甚少。在这项研究中，首次在 中鉴定出 23 个基因，具有 MtN3/唾液保守结构域，并通过系统发育树分为四个亚科。相同亚家族成员具有相似的基因结构和基序。在启动子区域发现了与糖和环境胁迫相关的多个 元件。此外，通过种内共线性分析发现 21 个基因定位于 11 条染色体上，存在 2 对同源基因对。表达谱分析结果表明 具有组织特异性。研究还表明 和 对低温和氧化胁迫有响应。此外，亚细胞定位研究表明 和 定位于细胞膜上。这项研究为深入阐明 基因的糖转运机制及其在应对非生物胁迫中的功能作用提供了重要线索。
