National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Xi'an 710119, China.
Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi'an 710119, China.
Int J Mol Sci. 2023 Oct 15;24(20):15202. doi: 10.3390/ijms242015202.
Basic leucine zipper (bZIP) transcription factors (TFs) are one of the largest families involved in plant physiological processes such as biotic and abiotic responses, growth, and development, etc. In this study, 66 members of the bZIP family were identified in , which were divided into 10 groups based on their phylogenetic relationships with . A structural analysis of revealed significant intron-exon differences among . A total of 63 bZIP genes were distributed across 16 chromosomes in . The tissue-specific and germination stage expression patterns of were based on RNA-seq. Stress-responsive expression analysis revealed that partial were highly expressed under low temperatures, wounding, oxidative stress, and GA treatments. Furthermore, subcellular localization studies indicated that was localized in the nucleus. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays suggested that could interact with multiple . The results of this study provide insightful data regarding bZIP TF as one of the stress response regulators in , while providing a theoretical basis for transgenic and functional studies of the bZIP gene family in .
基本亮氨酸拉链 (bZIP) 转录因子 (TF) 是参与植物生理过程的最大家族之一，例如生物和非生物响应、生长和发育等。在本研究中，在 中鉴定了 66 个 bZIP 家族成员，根据它们与 的系统发育关系将其分为 10 组。对 的结构分析表明 之间存在显著的内含子-外显子差异。总共 63 个 bZIP 基因分布在 的 16 条染色体上。根据 RNA-seq 分析了 的组织特异性和萌发阶段表达模式。胁迫响应表达分析表明，部分 在低温、创伤、氧化应激和 GA 处理下高度表达。此外，亚细胞定位研究表明 定位于细胞核。酵母双杂交 (Y2H) 和双分子荧光互补 (BiFC) 测定表明 可以与多个 相互作用。本研究的结果为 bZIP TF 作为 中应激反应调节剂之一提供了有见地的数据，同时为 中 bZIP 基因家族的转基因和功能研究提供了理论基础。
