Paris-Saclay Institute of Neurosciences (NeuroPSI), University of Paris-Saclay, CNRS, F-91400 Saclay, France.
Cells. 2022 Sep 1;11(17):2725. doi: 10.3390/cells11172725.
Peripheral adiponectin acts on the hypothalamus to inhibit energy expenditure and increase food intake through its receptors AdipoR1 and adipoR2. The hypothalamic expression of adiponectin is poorly documented. We hypothesize that whether hypothalamic adiponectin is confirmed, its expression and secretion could be regulated as peripheral adiponectin. Thus, in the present work, we aim to determine whether adiponectin is expressed in the hypothalamus and in two neuronal cell lines and investigate the potential mechanisms regulating its neuronal expression. Using immunohistochemistry, we show that adiponectin is expressed in the mediobasal hypothalamic neurons of mice. Adiponectin expression is also evidenced in two neuronal cell lines mHypo POMC (an adult mouse hypothalamic cell line) and SH-SY5Y (human neuroblastoma). The neuronal expression of adiponectin is increased in response to rosiglitazone treatment (a PPARγ agonist) and FGF21 and is decreased in insulin-resistant neurons. Furthermore, we show that adiponectin expressed by mHypo POMC neurons is secreted in a culture medium. Adiponectin also diminished the resistin-induced IL6 expression in SIMA9 cells, a microglia cell line. In conclusion, we evidenced the hypothalamic expression of adiponectin and its regulation at the neuronal level.
外周脂联素通过其受体 AdipoR1 和 adipoR2 作用于下丘脑，抑制能量消耗并增加食物摄入。脂联素在下丘脑的表达尚未得到充分证实。我们假设，无论是否证实下丘脑存在脂联素，其表达和分泌都可能像外周脂联素一样受到调节。因此，在本研究中，我们旨在确定脂联素是否在下丘脑和两种神经元细胞系中表达，并探讨调节其神经元表达的潜在机制。通过免疫组织化学，我们显示脂联素在小鼠的中脑基底部下丘脑神经元中表达。脂联素的表达也在两种神经元细胞系 mHypo POMC（一种成年小鼠下丘脑细胞系）和 SH-SY5Y（人神经母细胞瘤）中得到证实。脂联素的神经元表达在罗格列酮（PPARγ 激动剂）和 FGF21 处理时增加，在胰岛素抵抗神经元中减少。此外，我们还表明，mHypo POMC 神经元表达的脂联素在培养基中被分泌。脂联素还降低了 SIMA9 细胞（一种小胶质细胞系）中抵抗素诱导的 IL6 表达。总之，我们证实了下丘脑脂联素的表达及其在神经元水平的调节。
