Department of Bioscience, Faculty of Life Science, Okayama University of Science, Okayama 700-0005, Japan.
Wakayama Shin-ai Junior and Senior High School, Wakayama 640-8151, Japan.
Int J Environ Res Public Health. 2022 Aug 23;19(17):10474. doi: 10.3390/ijerph191710474.
Hexanoyl-lysine (HEL), 8-hydroxy-2'deoxyguanosine (8-OHdG), and dityrosine (DT) have served as potential biomarkers for detecting oxidative modified lipids, DNA, and proteins in biological samples, respectively. Whether regular higher levels of consumption of vegetables/fruit (V/F) would decrease oxidative modification of these biomolecules in the body remain unelucidated. To examine the association of regular V/F consumption with the generation of these reactive oxygen species-induced biomarkers, this study evaluated V/F consumption in a school-based sample of teenaged girls (mean age 15.6 ± 1.7 years, = 103), and quantified the formation of oxidative stress biomarkers in their urine. Only 19.4% and 23.3% of participants reported that they consumed the recommended daily amount of vegetables and fruits, respectively. Individuals who consumed lower levels of fruit (<100g/day) or vegetables (<250g/day) had significantly higher HEL excretion in their urine than those who consumed higher levels of fruit (≥100g/day) ( < 0.05) or vegetables (≥250g/day) ( = 0.057). The results of a multiple regression analysis showed that vegetable consumption was an important inhibiting factor of early lipid peroxidation measured as HEL in urine, independent of various confounders (β = - 0.332, < 0.05). The findings suggest that relatively higher consumption of vegetables would help in the prevention of early lipid peroxidation in adolescents.
己酰赖氨酸 (HEL)、8-羟基-2′-脱氧鸟苷 (8-OHdG) 和二酪氨酸 (DT) 分别作为检测生物样本中氧化修饰脂质、DNA 和蛋白质的潜在生物标志物。然而，定期摄入更多的蔬菜/水果（V/F）是否会降低体内这些生物分子的氧化修饰程度仍不清楚。为了研究定期摄入 V/F 与这些活性氧诱导生物标志物产生之间的关联，本研究评估了青少年女孩（平均年龄 15.6±1.7 岁，n=103）基于学校的样本中 V/F 的摄入情况，并定量检测了尿液中氧化应激生物标志物的形成情况。只有 19.4%和 23.3%的参与者分别报告称他们摄入了推荐的每日蔬菜和水果量。与摄入更高水平水果（≥100g/天）或蔬菜（≥250g/天）的个体相比，摄入较低水平水果（<100g/天）或蔬菜（<250g/天）的个体尿液中 HEL 排泄量明显更高（ < 0.05）或蔬菜（≥250g/天）（=0.057）。多元回归分析的结果表明，蔬菜的摄入量是尿液中早期脂质过氧化的一个重要抑制因素，独立于各种混杂因素（β=-0.332，<0.05）。研究结果表明，相对较高的蔬菜摄入量有助于预防青少年的早期脂质过氧化。
