School of Food Science and Engineering, Harbin Institute of Technology, No. 73 Huanghe Road, Nangang district, Harbin, China.
Food Funct. 2015 Jun;6(6):1984-91. doi: 10.1039/c5fo00438a.
Sulforaphane (SFN), which is an isothiocyanate (ITC) that is found in cruciferous vegetables, has received considerable attention because of its beneficial effects. In this study, the protection by SFN in the lysophosphatidylcholine (LPC)-induced injury of human vascular endothelial EA.hy.926 cells was investigated. ROS intensity was obtained by fluorescence microscopic imaging. Levels of MDA, GSH and the activity of SOD were determined spectrophotometrically. Expressions of GST, GSH-Px, TrxR and Nrf-2 proteins were measured by western blotting analysis. SFN largely decreased ROS production, similar to vitamin E. The MDA level was decreased by SFN to a level that was comparable to the negative group. Incubation with 0.5, 1.25, 2.5 μmol L(-1) SFN for 24 h restored the activity of SOD by 58%, 64%, and 123%, respectively. SOD activities were individually increased by 53%, 97%, 103% after treatment with 2.5 μmol L(-1) SFN for 12 h, 24 h, and 48 h, respectively. SFN restored and up-regulated the expressions of GST, GSH-Px and TrxR both in dose- and time-dependent ways. Although VE presents comparable induction of phase 2 enzymes as 1.25 μmol L(-1) SFN, it cannot induce the translocation of Nrf-2 to the nucleus. SFN protected the injury of vascular endothelial cell by LPC by enhancing anti-oxidative capabilities mediated by Nrf-2 translocation.
萝卜硫素（SFN）是十字花科蔬菜中含有的异硫氰酸盐（ITC），因其有益作用而受到广泛关注。本研究探讨了 SFN 对溶血磷脂酰胆碱（LPC）诱导的人血管内皮 EA.hy.926 细胞损伤的保护作用。通过荧光显微镜成像获得 ROS 强度。分光光度法测定 MDA、GSH 和 SOD 活性。Western blot 分析测定 GST、GSH-Px、TrxR 和 Nrf-2 蛋白的表达。SFN 可显著降低 ROS 产生，与维生素 E 相似。SFN 可降低 MDA 水平，使其接近阴性对照组。孵育 0.5、1.25、2.5μmol/L SFN 24 h 可分别使 SOD 活性恢复 58%、64%和 123%。用 2.5μmol/L SFN 处理 12 h、24 h 和 48 h 后，SOD 活性分别单独增加了 53%、97%和 103%。SFN 以剂量和时间依赖的方式恢复和上调 GST、GSH-Px 和 TrxR 的表达。尽管 VE 与 1.25μmol/L SFN 具有相当的诱导相 2 酶的作用，但它不能诱导 Nrf-2 向核内易位。SFN 通过增强 Nrf-2 易位介导的抗氧化能力来保护 LPC 引起的血管内皮细胞损伤。
