Zheng X M, Moncollin V, Egly J M, Chambon P
Cell. 1987 Jul 31;50(3):361-8. doi: 10.1016/0092-8674(87)90490-9.
A general transcription factor (BTF3) has been purified from HeLa whole cell extracts and shown to be required for accurate initiation of transcription from the adenovirus-2 major late promoter (Ad2MLP) and other RNA polymerase class B promoters. We show that purified BTF3 (27 kd) binds to RNA polymerase B (II), forming a complex that is transcriptionally active. We found no evidence that purified BTF3 interacts with DNA or is required for the formation of the stable preinitiation complex.
一种通用转录因子（BTF3）已从HeLa全细胞提取物中纯化出来，并被证明是腺病毒2型主要晚期启动子（Ad2MLP）和其他B类RNA聚合酶启动子精确起始转录所必需的。我们发现，纯化的BTF3（27千道尔顿）与RNA聚合酶B（II）结合，形成一种具有转录活性的复合物。我们没有发现证据表明纯化的BTF3与DNA相互作用或对稳定的起始前复合物的形成是必需的。
