Reinberg D, Roeder R G
J Biol Chem. 1987 Mar 5;262(7):3310-21.
Two general transcription factors (IIE and IIB) (TF) were purified from HeLa cell nuclear extracts and shown to be absolutely required, along with two additional factors (IIA and IID) and RNA polymerase II, for specific transcription initiation at the adenovirus major late promoter. TFIIB and TFIIE were also required, in addition to TFIIA, TFIID, RNA polymerase II, and the adenovirus 2 major late promoter, for the formation of a (preinitiation) complex that could initiate transcription (upon addition of nucleoside triphosphates) in the presence of heparin concentrations which inhibited the action of unbound factors. Glycerol gradient analyses indicated independent interactions of TFIIE with TFIIB and with the purified RNA polymerase II, but not with RNA polymerase III. Transcription factors IIB and IIE were also shown to be required for specific initiation of transcription from several cellular and viral class II promoters.
从HeLa细胞核提取物中纯化出两种通用转录因子（IIE和IIB），结果表明，它们与另外两种因子（IIA和IID）以及RNA聚合酶II一起，是腺病毒主要晚期启动子特异性转录起始绝对必需的。除了TFIIA、TFIID、RNA聚合酶II和腺病毒2主要晚期启动子外，TFIIB和TFIIE也是形成（预起始）复合物所必需的，该复合物在存在抑制未结合因子作用的肝素浓度下（加入核苷三磷酸后）能够起始转录。甘油梯度分析表明，TFIIE与TFIIB以及纯化的RNA聚合酶II存在独立相互作用，但与RNA聚合酶III没有相互作用。转录因子IIB和IIE也被证明是几种细胞和病毒II类启动子特异性转录起始所必需的。
