辅助生殖技术后受孕子代的DNA甲基化：系统评价与Meta分析

DNA Methylation in Offspring Conceived after Assisted Reproductive Techniques: A Systematic Review and Meta-Analysis.

作者信息

Cannarella Rossella, Crafa Andrea, Mongioì Laura M, Leggio Loredana, Iraci Nunzio, La Vignera Sandro, Condorelli Rosita A, Calogero Aldo E

机构信息

Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy.

Department of Biomedical and Biotechnological Sciences (BIOMETEC), University of Catania, Torre Biologica, 95125 Catania, Italy.

出版信息

J Clin Med. 2022 Aug 28;11(17):5056. doi: 10.3390/jcm11175056.

: In the last 40 years, assisted reproductive techniques (ARTs) have emerged as potentially resolving procedures for couple infertility. This study aims to evaluate whether ART is associated with epigenetic dysregulation in the offspring. . To accomplish this, we collected all available data on methylation patterns in offspring conceived after ART and in spontaneously conceived (SC) offspring. We extracted 949 records. Of these, 50 were considered eligible; 12 were included in the quantitative synthesis. Methylation levels of CCCTC-binding factor 3 (CTCF3) were significantly lower in the ART group compared to controls (SMD -0.81 (-1.53; -0.09), I = 89%, = 0.03). In contrast, CCCTC-binding factor 6 (CTCF6), (), (), and () were not differently methylated in ART vs. SC offspring. : The methylation pattern of the offspring conceived after ART may be different compared to spontaneous conception. Due to the lack of studies and the heterogeneity of the data, further prospective and well-sized population studies are needed to evaluate the impact of ART on the epigenome of the offspring.

在过去40年里，辅助生殖技术（ARTs）已成为解决夫妇不孕问题的潜在手段。本研究旨在评估ART是否与后代的表观遗传失调有关。为此，我们收集了所有关于ART受孕后代和自然受孕（SC）后代甲基化模式的可用数据。我们提取了949条记录。其中，50条被认为符合条件；12条被纳入定量综合分析。与对照组相比，ART组中CCCTC结合因子3（CTCF3）的甲基化水平显著降低（标准化均值差-0.81（-1.53；-0.09），I² = 89%，P = 0.03）。相比之下，ART后代与SC后代中CCCTC结合因子6（CTCF6）、（此处原文括号内容缺失，无法准确翻译）、（此处原文括号内容缺失，无法准确翻译）和（此处原文括号内容缺失，无法准确翻译）的甲基化没有差异。：与自然受孕相比，ART受孕后代的甲基化模式可能不同。由于缺乏研究以及数据的异质性，需要进一步进行前瞻性且样本量充足的人群研究，以评估ART对后代表观基因组的影响。