Križman Mitja, Jakše Jernej
Laboratory of Food Chemistry, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia.
Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia.
Plants (Basel). 2022 Aug 29;11(17):2239. doi: 10.3390/plants11172239.
Wild populations from the region of Istria have been subjected to a genetic and chemical study. Headspace-gas chromatography analysis of volatile secondary metabolites and PCR-RFLP analysis of the ribosomal DNA internal transcribed spacer region has been chosen to analyze the chemical and genetic traits of single plants, respectively. Large intrapopulation and interpopulation differences have been observed in both chemical profiles and restriction patterns of PCR products. The data from chemical and genetic analyses were pooled and used to assess allele frequencies of three putative genetic loci on individual populations. The pooled allele frequencies were used to determine interpopulation distances for phenogram reconstruction. The combined use of chemical and genetic datasets for genetic variation analysis proved to be a more comprehensive approach for such a study, compared to the use of single datasets, even using such relatively simple analytical tools.
伊斯特拉地区的野生种群已接受了一项基因和化学研究。分别选用了挥发性次生代谢物的顶空气相色谱分析和核糖体DNA内部转录间隔区的PCR-RFLP分析来分析单株植物的化学和遗传特征。在化学图谱和PCR产物的限制性模式中均观察到了较大的种群内和种群间差异。将化学和遗传分析的数据汇总起来，用于评估各个种群中三个假定基因座的等位基因频率。汇总的等位基因频率用于确定用于构建系统树状图的种群间距离。与使用单个数据集相比，即使使用相对简单的分析工具，联合使用化学和遗传数据集进行遗传变异分析被证明是一种更全面的研究方法。
