Huang Wenyue, Wang Shaoxing, Zhang Shifan, Li Fei, Zhang Hui, Sun Rifei, Zhang Shujiang, Li Guoliang
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, Nandajie No. 12, Haidian District, Beijing 100081, China.
College of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, Changjiang West Road, No. 130, Hefei 230036, China.
Plants (Basel). 2022 Aug 30;11(17):2248. doi: 10.3390/plants11172248.
is one of the most important leafy vegetables worldwide, and has a long history of cultivation. However, it has not been possible to completely control the damage of turnip mosaic virus (TuMV), a serious virus in , to production. In this study, the genome-wide identification and expression detection of eIF family genes from in response to TuMV resistance were analyzed, including the identification of eIF family genes, chromosomal distribution, three-dimensional (3D) structure and sequence logo analyses, and the expression characterization as well as differential metabolite analysis of eIF family genes in resistant/susceptible lines, which may further prove the whole-genome tripling (WGT) event in evolution and provide evidence for the functional redundancy and functional loss of multicopy eIF genes in evolution. A qRT-PCR analysis revealed that the relative expressions of eIF genes in a susceptible line (80461) were higher than those in a resistant line (80124), which may prove that, when TuMV infects host plants, the eIF genes can combine with the virus mRNA 5' end cap structure and promote the initiation of virus mRNA translation in the susceptible line. In addition, the metabolite substances were detected, the differences in metabolites between disease-resistant and disease-susceptible plants were mainly manifested by altered compounds such as flavonoids, jasmonic acid, salicylic acid, ketones, esters, etc., which inferred that the different metabolite regulations of eIF family genes and reveal the resistance mechanisms of eIF genes against TuMV in brassica crops. This study may lay a new theoretical foundation for revealing eIF family gene resistance to TuMV in , as well as advancing our understanding of virus-host interactions.
是全球最重要的叶菜类蔬菜之一，有着悠久的种植历史。然而，一直无法完全控制芜菁花叶病毒（TuMV）对其生产造成的损害，TuMV是一种严重危害的病毒。在本研究中，分析了响应TuMV抗性的芸苔属植物eIF家族基因的全基因组鉴定和表达检测，包括eIF家族基因的鉴定、染色体分布、三维（3D）结构和序列标识分析，以及抗性/感病品系中eIF家族基因的表达特征和差异代谢物分析，这可能进一步证明芸苔属植物进化过程中的全基因组三倍化（WGT）事件，并为多拷贝eIF基因在进化中的功能冗余和功能丧失提供证据。qRT-PCR分析表明，感病品系（80461）中eIF基因的相对表达高于抗病品系（80124），这可能证明，当TuMV感染寄主植物时，eIF基因可与病毒mRNA 5'端帽结构结合，促进感病芸苔属植物品系中病毒mRNA翻译的起始。此外，还检测了代谢物质，抗病和感病植物之间代谢物的差异主要表现为黄酮类、茉莉酸、水杨酸、酮类、酯类等化合物的变化，这推断出eIF家族基因的不同代谢调控方式，并揭示了芸苔属作物中eIF基因对TuMV的抗性机制。本研究可能为揭示芸苔属植物中eIF家族基因对TuMV的抗性奠定新的理论基础，同时增进我们对病毒-寄主相互作用的理解。
