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菲利恩斯卡亚湾(波罗的海)微藻的抗氧化和抗菌活性

Antioxidant and Antimicrobial Activity of Microalgae of the Filinskaya Bay (Baltic Sea).

作者信息

Shevelyuhina Alexandra, Babich Olga, Sukhikh Stanislav, Ivanova Svetlana, Kashirskih Egor, Smirnov Vitaliy, Michaud Philippe, Chupakhin Evgeny

机构信息

Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, Kaliningrad 236016, Russia.

Natural Nutraceutical Biotesting Laboratory, Kemerovo State University, Krasnaya Street 6, Kemerovo 650043, Russia.

出版信息

Plants (Basel). 2022 Aug 31;11(17):2264. doi: 10.3390/plants11172264.

Abstract

Microalgae are rich in proteins, carbohydrates, lipids, polyunsaturated fatty acids, vitamins, pigments, enzymes, and other biologically active substances. This research aimed to study the composition and antioxidant and antimicrobial activity of proteins, lipids, and carbohydrates of microalgae found in the Filinskaya Bay of the Kaliningrad region. The chemical composition of and microalgae biomass was studied. Ultrasound was used to isolate valuable components of microalgae. It was found that microalgae are rich in protein and contain lipids and reducing sugars. To confirm the accuracy of the determination, the protein content was measured using two methods (Kjeldahl and Bradford). Protein content in and microalgae samples did not differ significantly when measured using different methods. Protein extraction by the Kjeldahl method was found to be 63.27% for microalgae samples and 60.11% for microalgae samples. Protein content in samples was 63.46%, compared to 60.07% in samples, as determined by the Bradford method. Lipids were 8.0-8.2 times less abundant than protein in both types of microalgae samples. It was determined that the content of reducing sugars in the samples of the studied microalgae was 5.9 times less than the protein content. The presence of biological activity (antioxidant) in proteins and lipids obtained from biomass samples of the studied microscopic algae was established, which opens up some possibilities for their application in the food, chemical, and pharmaceutical industries (as enzymes, hormones, vitamins, growth substances, antibiotics, and other biologically active compounds).

摘要

微藻富含蛋白质、碳水化合物、脂质、多不饱和脂肪酸、维生素、色素、酶及其他生物活性物质。本研究旨在探究加里宁格勒地区菲利恩斯卡亚湾发现的微藻中蛋白质、脂质和碳水化合物的组成及其抗氧化和抗菌活性。对两种微藻生物质的化学成分进行了研究。采用超声法分离微藻的有价值成分。结果发现微藻富含蛋白质,且含有脂质和还原糖。为证实测定的准确性,使用两种方法(凯氏定氮法和布拉德福德法)测定蛋白质含量。用不同方法测定时,两种微藻样品中的蛋白质含量无显著差异。凯氏定氮法提取的两种微藻样品的蛋白质含量分别为63.27%和60.11%。用布拉德福德法测定,两种微藻样品中的蛋白质含量分别为63.46%和60.07%。两种微藻样品中脂质的含量均比蛋白质少8.0 - 8.2倍。经测定,所研究微藻样品中还原糖的含量比蛋白质含量少5.9倍。已确定从所研究的微藻生物质样品中获得的蛋白质和脂质具有生物活性(抗氧化),这为它们在食品、化工和制药行业(作为酶、激素、维生素、生长物质、抗生素及其他生物活性化合物)的应用开辟了一些可能性。

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