Marrez Diaa A, Naguib Mohamed M, Sultan Yousef Y, Higazy Aziz M
Food Toxicology and Contaminants Department, National Research Centre, Cairo, Egypt.
Microbiology Department, Faculty of Agriculture, Cairo University, Cairo, Egypt.
Heliyon. 2019 Mar 27;5(3):e01404. doi: 10.1016/j.heliyon.2019.e01404. eCollection 2019 Mar.
This study was conducted to evaluate the antimicrobial activities and cytotoxicity of both crude extracts of and their fractions. cytotoxicity assay against human hepatocellular carcinoma (HepG2), colon cancer (HCT116) and breast cancer (MCF7) cell line was monitored. The highest inhibition was observed using diethyl ether crude extract (DEE) recording between 12.5 and 19.5 mm inhibition zone against all tested bacteria and between 8.7 and 18.3 mm against tested fungi. The highest anticancer effect of DEE was observed at IC against HCT116 and HepG2 cell lines using just 24.6 and 42.8 μg ml, respectively. While, high concentration, 93.8 μg ml, was required to exhibit its effect against MCF7. Column chromatography technique was used to separate DEE crude extract to its main components using 7 different mobile phases. Fractions F1 and F7 were the highest fractions that had antimicrobial activity against tested bacteria and fungi. High IC > 80 μg ml were required to exhibit anticancer activity at IC against the tested cancer cell lines. The main compounds responsible for the bioactivity were identified using GC-MS, nonadecane and butylated hydroxytoluene in F1 and 9-octadecadienoic acid and quercetin 7,3',4'-trimethoxy in F7 were identified. The current study highlights the potential use of . extract and their fractions as a source of antimicrobial and anticancer compounds.
本研究旨在评估[提取物名称]的粗提物及其馏分的抗菌活性和细胞毒性。监测了其对人肝癌细胞系（HepG2）、结肠癌细胞系（HCT116）和乳腺癌细胞系（MCF7）的细胞毒性测定。使用二乙醚粗提物（DEE）观察到最高抑制率，对所有测试细菌的抑菌圈直径在12.5至19.5毫米之间，对测试真菌的抑菌圈直径在8.7至18.3毫米之间。仅分别使用24.6和42.8μg/ml的DEE时，观察到其对HCT116和HepG2细胞系的最高抗癌效果。而对MCF7发挥作用则需要93.8μg/ml的高浓度。采用柱色谱技术，使用7种不同的流动相将DEE粗提物分离为其主要成分。馏分F1和F7是对测试细菌和真菌具有抗菌活性的最高馏分。在IC50时对测试癌细胞系表现出抗癌活性需要>80μg/ml的高IC50。使用气相色谱-质谱联用仪（GC-MS）鉴定了负责生物活性的主要化合物，在F1中鉴定出了十九烷和丁基化羟基甲苯，在F7中鉴定出了9-十八碳二烯酸和槲皮素7,3',4'-三甲氧基。本研究突出了[提取物名称]提取物及其馏分作为抗菌和抗癌化合物来源的潜在用途。
