Effect of the Application of Hydrolysate of Extracted by Different Techniques on the Growth of × .

The extraction method used to obtain biologically active compounds from microalgal biomass may affect the biostimulant capacity of the microalgae. The objective of this assay was to determine the most efficient extraction method to release the active components of the biomass of (). Plantlets of × were grown in a greenhouse and five treatments were applied: C-application with water; M-application with untreated microalgae; M-US-application with microalgae treated with ultrasound; M-USHY-application with microalgae treated with ultrasound and enzymatic hydrolysis; and M-USHYAU-application with microalgae treated with ultrasound, enzymatic hydrolysis, and autoclaving. All microalgae treatments increased shoot number and stem and plant diameter. The US-treated biomass increased the inflorescence of the plant significantly compared to the control. To extract bioactive compounds from eukaryotic microalgae for plant biostimulating purposes, the US-treatment (or any other method damaging the plasma membrane) of microalgae cell is, or seems to be, suitable.. Macronutrient content in leaves was not affected by the microalgae treatment, except for K.