Carillo Petronia, Pannico Antonio, Cirillo Chiara, Ciriello Michele, Colla Giuseppe, Cardarelli Mariateresa, De Pascale Stefania, Rouphael Youssef
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Via Vivaldi 43, 81100 Caserta, Italy.
Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy.
Plants (Basel). 2022 Sep 5;11(17):2321. doi: 10.3390/plants11172321.
Protein hydrolysates (PHs) are a prominent category of plant biostimulants, mainly constituted of amino acids, oligopeptides and polypeptides, obtained by partial hydrolysis of animal or plant protein sources. Despite scientific evidence supporting the biostimulant action of PHs on vegetables, the morphological, physiological, and shelf-life performances underlying the PH action on cut flowers are still poorly explored. Accordingly, the aim of this research is to assess the effects of three commercial biostimulants, one animal PH (PH A, Hicure) and two plant PHs (PH V1, Trainer and PH V2, Vegamin©), on two chrysanthemum () cultivars (Pinacolada and Radost). In both cultivars, only the plant-derived PH (V1 and V2) treatments recorded significantly higher fresh plant biomass than the control (on average +18%, in both cultivars). The foliar application of the vegetal-derived PHs but not the animal one, particularly in Pinacolada, improved the status of plants, stimulating stem elongation and the apical flower diameter. In Pinacolada, applications with PH V1 resulted in a significant increase in nitrate and P concentration in leaves and Ca content in flowers compared with the control (+43%, +27%, and +28% for nitrate, P, and Ca, respectively). In Radost, PH A and PH V2 applications caused a significant reduction in nitrate concentration in both leaves and flowers compared with the control. One week after harvest, in both cultivars, PH A applications caused flower stems to wilt faster than the control. In contrast, plants treated with PH V1 revealed significantly slower flower stem senescence compared to the control. Flower wilting during vase life was correlated to a decrease in the K-to-Na ratio in flowers due to an inability to transport K to the flowers from the leaves rather than an increase in Na in the flowers themselves.
蛋白质水解物（PHs）是植物生物刺激剂的一个重要类别，主要由氨基酸、寡肽和多肽组成，通过对动物或植物蛋白来源进行部分水解获得。尽管有科学证据支持PHs对蔬菜的生物刺激作用，但PHs对切花的形态、生理和货架期表现的影响仍未得到充分研究。因此，本研究的目的是评估三种商业生物刺激剂，一种动物源PH（PH A，Hicure）和两种植物源PHs（PH V1，Trainer和PH V2，Vegamin©）对两个菊花（）品种（Pinacolada和Radost）的影响。在两个品种中，只有植物源PH（V1和V2）处理的新鲜植物生物量显著高于对照（两个品种平均均增加18%）。叶面施用植物源PHs而非动物源PHs，特别是在Pinacolada品种中，改善了植物的状况，促进了茎的伸长和顶端花直径的增大。在Pinacolada品种中，与对照相比，施用PH V1导致叶片中硝酸盐和磷浓度以及花中钙含量显著增加（硝酸盐、磷和钙分别增加43%、27%和28%）。在Radost品种中，施用PH A和PH V2导致叶片和花中的硝酸盐浓度均显著低于对照。收获后一周，在两个品种中，施用PH A导致花茎比对照更快枯萎。相比之下，与对照相比，用PH V1处理的植物显示花茎衰老明显较慢。瓶插期的花枯萎与花中钾钠比的降低有关，这是由于无法将钾从叶片运输到花中，而不是花中钠含量的增加。
