Simultaneous Determination and Pharmacokinetics Study of Three Triterpenes from L. in Rats by UHPLC-MS/MS.

A selective and rapid ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was established and validated for the determination of ziyuglycoside I, 3,19-dihydroxyurs-12-en-28-oic-acid 28--d-glucopyranosyl ester, and pomolic acid in rats after the oral administration of ziyuglycoside I, 3,19-dihydroxyurs-12-en-28-oic-acid 28--d-glucopyranosyl ester, pomolic acid, and L. extract. The separation was carried out on an ACQUITY UPLCHSS T column (2.1 mm × 100 mm, 1.8 μm), using methanol and 5 mmol/L ammonium acetate water as the mobile phase. The three compounds were quantified using the multiple reaction monitoring mode with the electrospray ion source in both the positive and negative mode. Liquid-liquid extraction was applied to the plasma sample preparation. Bifendate was selected as the internal standard. The intra-day and inter-day precision and the accuracy of the method were all within receivable ranges. The lower limit of quantification of ziyuglycoside I, 3,19-dihydroxyurs-12-en-28-oic-acid 28--d-glucopyranosyl ester, and pomolic acid were 6.50, 5.75, and 2.63 ng/mL, respectively. The extraction recoveries of analytes in rat plasma ranged from 83 to 94%. The three components could be rapidly absorbed into the blood (, 1.4-1.6 h) both in the single-administration group or extract group, but the first peak of PA occurred at 0.5 h and the second peak at 4-5 h in the extract. Three compounds were eliminated relatively slowly (, 7.3-11 h). The research was to establish a rapid, sensible, and sensitive UHPLC-MS/MS method using the multi-ion mode for multi-channel simultaneous mensuration pharmacokinetics parameters of three compounds in rats after oral administration of extract. This study found, for the first time, differences in the pharmacokinetic parameters of the three compounds in the monomer compounds and extract administration, which preliminarily revealed the transformation and metabolism of the three compounds in vivo.