Wang Liqian, Gan Chunli, Wang Zhibin, Liu Lu, Gao Mingjie, Li Qian, Yang Chunjuan
Department of Pharmaceutical Analysis and Analytical Chemistry, College of Pharmacy, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin 150081, Heilongjiang, China.
Department of Medicinal Chemistry and Natural Medicine Chemistry, College of Pharmacy, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin 150081, Heilongjiang, China.
Molecules. 2017 Jun 4;22(6):934. doi: 10.3390/molecules22060934.
L. is commonly used as a spice and flavoring agent. Diterpenes are the main active compounds of . An Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry (UHPLC-ESI-MS/MS) method was developed for the determination of carnosol, rosmanol, and carnosic acid isolated from in rat plasma, and applied to a pharmacokinetic study after oral administration of extract. Sample preparation involved a liquid-liquid extraction of the analytes with ethyl acetate. Butylparaben was employed as an internal standard (I.S.). Chromatographic separation was carried out on a C column (ACQUITY UPLC HSS T3, 1.8 μm, 2.1 mm × 100 mm) with a gradient system consisting of the mobile phase solution A (0.1% formic acid in water) and solution B (acetonitrile) at the flow rate of 0.3 mL/min. The quantification was obtained using multiple reaction monitoring (MRM) mode with electrospray ionization (ESI). The UHPLC-MS/MS assay was validated for linearity, accuracy, precision, extraction recovery, matrix effect and stability. This study described a simple, sensitive and validated UHPLC-MS/MS method for the simultaneous determination of three diterpene compounds in rat plasma after oral administration of extract, and investigated on their pharmacokinetic studies as well.
L. 通常用作香料和调味剂。二萜类化合物是其主要活性成分。建立了一种超高效液相色谱 - 串联质谱法（UHPLC - ESI - MS/MS），用于测定从大鼠血浆中分离出的鼠尾草酸、迷迭香酚和鼠尾草酚，并将其应用于提取物口服给药后的药代动力学研究。样品制备包括用乙酸乙酯对分析物进行液 - 液萃取。对羟基苯甲酸丁酯用作内标（I.S.）。色谱分离在C柱（ACQUITY UPLC HSS T3，1.8μm，2.1mm×100mm）上进行，流动相为溶液A（0.1%甲酸水溶液）和溶液B（乙腈）组成的梯度系统，流速为0.3mL/min。采用电喷雾电离（ESI）的多反应监测（MRM）模式进行定量。该UHPLC - MS/MS测定法在线性、准确性、精密度、提取回收率、基质效应和稳定性方面进行了验证。本研究描述了一种简单、灵敏且经过验证的UHPLC - MS/MS方法，用于同时测定提取物口服给药后大鼠血浆中的三种二萜类化合物，并对其药代动力学进行了研究。
